Study on Ultra-Structural Numerical Changes in the Intestinal Mucosal Epithelial Cell Microvilli after Excessive Zinc Supplementation in Wistar Rat

Silpakorn University Science and Technology Journal Pub Date : 2018-07-01 DOI:10.22232/stj.2018.06.02.08

Tanushree Das, H. Lalramnghaki, K. Singh

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Abstract

The present study was aimed to study the ultra-structural numerical changes in the microvilli of intestinal mucosal epithelial cells (IMECs) after excessive Zn supplementation in wistar rats. In this study, three groups of rats were fed on an equicaloric sugar and fat rich semi-synthetic diet containing 20mg (control, group-I), 50mg (group-II) and 80mg Zn /kg (group-III) diet respectively for 4 months. The data of the study revealed an increase in surface area of intestinal absorption site through increase in the number and height of microvilli/cell in Zn concentration dependent manner. Further, the result of the study reveals the presence of higher Zn concentrations in IMECs (tissue) and higher absorption rates (HARs) of 14C-glucose (μmoles/g.5min) and amino acids namely 14C-L-alanine and 14C-L-isoleucine (μmoles/g.5min) in group-II and III than that of control group-I. Thus, high Zn supplementation for a longer period of time alters ultrastructure of intestinal mucosal epithelial cell microvilli and increase absorption of nutrients in wistar rats.

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过量补锌后Wistar大鼠肠黏膜上皮细胞微绒毛超微结构数值变化的研究

本研究旨在研究过量补锌后wistar大鼠肠黏膜上皮细胞(IMECs)微绒毛超微结构数值的变化。在本研究中，三组大鼠分别饲喂含有20mg(对照组，i组)、50mg (ii组)和80mg Zn /kg (iii组)的等量糖和脂肪丰富的半合成饲料，为期4个月。本研究数据显示，肠道吸收部位表面积的增加是通过微绒毛/细胞的数量和高度的增加，并呈锌浓度依赖性。此外，研究结果显示，与对照组相比，ii组和III组的imec(组织)中锌浓度更高，对14c -葡萄糖(μmol /g.5min)和14c - l -丙氨酸和14c - l -异亮氨酸(μmol /g.5min)的吸收率(HARs)更高。由此可见，长时间补充高锌可改变wistar大鼠肠黏膜上皮细胞微绒毛的超微结构，增加营养物质的吸收。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Silpakorn University Science and Technology Journal

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