CYTOLOGICAL STUDY ON RAT ISOLATED HEPATOCYTES AND POSSIBLE PROTECTION OF GINGER (EXTRACT AND NANOPARTICLES) AGAINST ACETAMINOPHEN TOXICITY

S. Abdelgayed, Alaa F. Bakr, O. El-tawil, A. Bakeer
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引用次数: 1

Abstract

Ginger is one of most important medicinal plants which used in variable diseases. Recently, ginger nanoparticles have been isolated from ginger extract and exhibited anti-apoptotic activity, hepatoprotective effect against alcohol and may effectively reduce colitis. Our study aimed to compare between the effect of ginger extract and ginger nanoparticles at concentration of 60 µg/ml against hepatotoxicity caused by acetaminophen (APAP; N-acetyl-para-aminophenol) at concentration of 0.1 mg/ml using primary isolated rat hepatocytes. Cytotoxicity was determined by assessing cell viability and leakage of cytosolic enzymes, such as (ALT& AST). Oxidative stress was investigated by measuring levels of MDA and catalase enzymes. The cytopathological lesions were detected by light microscope. Exposure of isolated rat hepatocytes to APAP caused cytotoxicity and oxidative injury, manifested by loss of cell viability and significant increase of enzymes leakages. Also, APAP caused progressive depletion of catalase content and increase in intracellular MDA accumulation, in addition to alteration in histological structures of the cells. Pretreatment of hepatocytes with either GE or GNPs ameliorated the hepatotoxicity, oxidative stress and enzymatic leakage induced by APAP. However, GNPs were more effective compared to ginger extract pre-treated hepatocytes.
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大鼠离体肝细胞的细胞学研究及生姜(提取物和纳米颗粒)对乙酰氨基酚毒性的可能保护作用
生姜是治疗多种疾病的重要药用植物之一。近年来,从生姜提取物中分离出生姜纳米颗粒,显示出抗凋亡活性、抗酒精保护肝和有效减轻结肠炎的作用。本研究旨在比较生姜提取物和浓度为60µg/ml的生姜纳米颗粒对对乙酰氨基酚(APAP;n -乙酰-对氨基酚)浓度为0.1 mg/ml,使用原代分离的大鼠肝细胞。通过评估细胞活力和胞质酶(alt和AST)的渗漏来确定细胞毒性。通过测定丙二醛和过氧化氢酶的水平来研究氧化应激。光镜下观察细胞病理病变。离体大鼠肝细胞暴露于APAP可引起细胞毒性和氧化损伤,表现为细胞活力丧失和酶泄漏显著增加。此外,APAP引起过氧化氢酶含量的逐渐减少和细胞内MDA积累的增加,以及细胞组织结构的改变。GE或GNPs预处理肝细胞均可改善APAP诱导的肝毒性、氧化应激和酶泄漏。然而,与生姜提取物预处理的肝细胞相比,GNPs更有效。
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