Identification and Assemblage Types of Giardia duodenalis from Patients in Thrace, Turkey

Sadiye Kaplan Kucuk, G. Akyildiz, R. Bircan, N. Yilmazer, A. Gargılı, S. Kar
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引用次数: 1

Abstract

Objective: Giardiasis is a common disease, and clinical forms can vary based on the assemblage types of the parasite. Detailed information on the subgenotypes may indicate the transmission routes and enlighten the gaps in the epidemiology of the disease. This study aims to reveal the occurrence of giardiasis in Thrace, Turkey, and assemblage types of Giardia duodenalis. Materials and Methods: In total, 573 stool samples taken from the individuals applied to Tekirdağ Central State Hospital in 2009, were examined by wet-mount and zinc sulfate floatation methods. Giardia-positive 26 samples and 64 samples taken from the individuals with gastrointestinal complaints were analyzed by nested PCR-RFLP to differentiate the assemblage types. Sequence analysis was employed for confirmation of assemblage types and subgenotypes. Results: Giardia spp. cysts were detected in 3.66% and 4.54% of the samples with wetmount and zinc sulphate floatation techniques respectively. A total of 27 samples were found positive by nested PCR-RFLP out of 90 samples. Fifteen samples were determined as assemblage A, 2 and 10 samples as B and B/E mix respectively. Sequence analysis showed that the latter assemblage (B/E mix) as A3-B3 mix. Conclusion: Fast identification techniques, namely zinc sulphate flotation can be used for screening stool samples in order to determine Giardia cysts with considerably high sensitivity and specificity. Based on this method, the occurrence rate of giardiasis was found as 4.54% in the studied group. DNA sequencing is necessary to distinguish assemblages and confirm the results of PCR-RFLP.
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土耳其色雷斯患者十二指肠贾第虫的鉴定和组合类型
目的:贾第虫病是一种常见的疾病,临床形式可根据寄生虫的组合类型而变化。亚基因型的详细信息可能指示传播途径,并启发疾病流行病学的空白。本研究旨在揭示贾第虫病在色雷斯、土耳其的发生和十二指肠贾第虫的聚集类型。材料和方法:采用湿法浮法和硫酸锌浮法对2009年在泰克尔达伊尔中央国家医院采集的573份粪便样本进行检测。采用巢式PCR-RFLP方法对26份贾第鞭毛虫阳性标本和64份胃肠道疾病患者标本进行分析,以区分其组合类型。序列分析确定了组合型和亚基因型。结果:湿法和硫酸锌法检出贾第鞭毛虫囊蚴的检出率分别为3.66%和4.54%。巢式PCR-RFLP检测90份样品中27份呈阳性。15个样品分别确定为组合A, 2个样品确定为组合B, 10个样品确定为组合B/E混合物。序列分析表明,后一组合(B/E混合)为A3-B3混合。结论:快速鉴定技术即硫酸锌浮选技术可用于粪便样品筛选,对贾第鞭毛虫囊肿的检测具有较高的敏感性和特异性。根据该方法,研究组贾第虫病发病率为4.54%。DNA测序是区分组合和确认PCR-RFLP结果的必要手段。
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