An Optimization Study for Chitosan Nanoparticles: Synthesis and Characterization

Abstract

Studies have been carried out to determine the optimum conditions for chitosan nanoparticles. Various formulations have been made which can affect the size and polydispersity index of the nanoparticles and the process variables have been investigated. These formulation and process variables are defined and optimized to obtain the smallest particle size. The concentration of chitosan polymer and crosslinker concentration were studied as formulation variables. Mixing speed, mixing time, pH, sunlight effect, sonication time and sonication power parameters were selected as process variables. In the experiments performed for one parameter all other parameters are kept constant. The optimum conditions were determined by the effect of formulation and process variables on particle size and polydispersity index. For characterization of chitosan nanoparticles; Zeta-Sizer, UV-Vis, FTIR and SEM analytical techniques were used. Optimum conditions found at 50 W, 5 min, 30-10 pulse adjustment with sonicator device, chitosan:TPP mass ratio 5:1, pH value of 5 and with sunlight. After the optimum conditions obtained BSA loading is performed and characterization studies carried out. It is believed that chitosan nanoparticles produced by optimum conditions determined as a result of the study can be used in many areas include as a drug delivery system in future studies.