The Protective effect of Crocin on Ovary Mast cells, Blood Vessels and Ovary, Serum Biochemical changes following Busulfan-induced Oxidative Stress in Mice.

Abstract

Aim: The purpose of this research was aimed to evaluate the protective effects of crocin, as an antioxidant agent on Mast cells, blood vessels and biochemical changes of ovary and serum in Busulfan-induced oxidative stress in Mice. Material and Methods: Thirty mature 6-8 weeks aged female NMRI mice in the weight of 22-25 g were randomly divided into 6 groups, and treated for 21 days. The control group only received solvent of Busulfan (BSF) (0.1 ml) intraperitoneally, and BSF group received only Busulfan (10 mg kg -1 , IP/single dose). The experimental groups no. 1, 2, 3 received BSF (10 mg kg -1 /single dose) with crocin (100, 200, 400 mg kg -1 /day, IP) and positive group only received crocin (400 mg kg -1 , IP/day). At the end of treatment period, animals were euthanized and left ovary were studied for Mast cells, ovary blood vessels, and Serum, and right ovary for biochemical evaluations. Data was subjected to one‒way analysis of variance (ANOVA) and Tukey to determine if significant difference (P≤0.01) existed among the observed results using SPSS. Results: Busulfan significantly (P≤0.01) increased mast cells and MDA, while decreased ovary blood vessels and SOD rate, significantly (P=0.000) in comparison to control group. However, crocin in all the used doses, especially in the dose of 200 mg kg -1 , significantly decreased the adverse effects of Busulfan. Conclusion: The results indicated that crocin can protect ovaries against Busulfan induced damages, and it can be considered as a suitable drug for reducing the toxic effects of Busulfan in chemotherapy.