Seed-borne mycoflora of important oilseeds of Nagaland

Anamika Debbarma, S. Banik
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Abstract

The present investigation was carried out to identify fungal infection of five oilseeds viz., rapeseed, mustard, soybean, sesame and perilla oilseeds via different seed health testing methods and seed germination. A total of six seed-borne fungi Aspergillus sp., Colletotrichum sp., Rhizopus sp., Penicillium sp., Nigrospora sp. and Trichoderma sp. were detected from the five oilseeds collected from local market of Medziphema, Nagaland. Amongst the seed health testing methods employed, PDA method was proved to be superior to blotter paper method and water-agar method. The fungal occurrence is more in PDA method with mean incidence of seed mycoflora 15.77%. The predominant fungus was observed to be Trichoderma sp. with 40.67% incidence and the least fungal incidence was of Nigrospora sp. (1.33%). Among the oilseeds, soybean seeds showed highest incidence of seed mycoflora (14.81%) and the lowest incidence of 4.24% was recorded from perilla seeds. The highest germination of oilseeds was recorded in PDA method (50.33%) followed by water-agar method (45.67%).
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那加兰邦重要油籽的种传菌群
采用不同的种子健康检测方法和种子萌发试验,对油菜、芥菜、大豆、芝麻和紫苏5种油籽进行了真菌侵染鉴定。从那加兰邦麦芝费马当地市场采集的5种油籽中共检出6种种传真菌曲霉、炭疽菌、根霉、青霉、黑孢菌和木霉。结果表明,PDA法优于吸墨纸法和水琼脂法。PDA法真菌发生率较高,种子菌群平均发生率为15.77%。真菌以木霉(Trichoderma sp.)为优势菌,发病率40.67%,黑孢菌(Nigrospora sp.)最低,发病率1.33%。油料种子中,大豆种子真菌发病率最高(14.81%),紫苏种子发病率最低(4.24%)。油籽发芽率以PDA法最高(50.33%),其次为水琼脂法(45.67%)。
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