Odontuya Davaadorj, H. Akatsuka, Yoshiki Yamaguchi, Chisa Okada, Masatoshi Ito, N. Fukunishi, Y. Sekijima, H. Ohnota, K. Kawai, Takahiro Suzuki, Takehito Sato, Yasuyuki Suzuki
{"title":"Impaired Autophagy in Retinal Pigment Epithelial Cells Induced from iPS Cells obtained from a Patient with Sialidosis","authors":"Odontuya Davaadorj, H. Akatsuka, Yoshiki Yamaguchi, Chisa Okada, Masatoshi Ito, N. Fukunishi, Y. Sekijima, H. Ohnota, K. Kawai, Takahiro Suzuki, Takehito Sato, Yasuyuki Suzuki","doi":"10.4172/2168-9296.1000188","DOIUrl":null,"url":null,"abstract":"Sialidosis type I patient-derived induced pluripotent stem cells (iPSCs) were generated from blood mononuclear cells. During embryoid body-like 3D culture, aggregates of patient-derived iPSCs were irregular in shape and had increased vacuoles filled with lipid droplets and cellular components such as damaged mitochondria. Retinal pigment epithelial cells induced from patient-derived iPSCs showed impaired autophagy flux with decreased formation of LC3 puncta. Sialidosis patient-derived iPSCs could provide a useful tool for investigating the mechanism of the autophagy/ lysosome-mediated degradation system.","PeriodicalId":9775,"journal":{"name":"Cell & developmental biology","volume":"1 1","pages":"1-7"},"PeriodicalIF":0.0000,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cell & developmental biology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/2168-9296.1000188","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
Sialidosis type I patient-derived induced pluripotent stem cells (iPSCs) were generated from blood mononuclear cells. During embryoid body-like 3D culture, aggregates of patient-derived iPSCs were irregular in shape and had increased vacuoles filled with lipid droplets and cellular components such as damaged mitochondria. Retinal pigment epithelial cells induced from patient-derived iPSCs showed impaired autophagy flux with decreased formation of LC3 puncta. Sialidosis patient-derived iPSCs could provide a useful tool for investigating the mechanism of the autophagy/ lysosome-mediated degradation system.
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