PERBANDINGAN KADAR FLAVONOID EKSTRAK ETANOL DAUN KELENGKENG (Dimocarpus logan L.) DENGAN VARIASI METODE EKSTRAKSI

Q3 Environmental Science Ekologia Bratislava Pub Date : 2022-04-27 DOI:10.33751/ekologia.v22i1.5042
Z. Rusli, Yulianita Yulianita, Elfrida Erawati Sitanggang
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Abstract

Longan plants (Dimocarpus logan L.) are generally used by the community only for their fruit. Longan leaves contain secondary metabolites such as flavonoids, polyphenols, saponins, and tannins. Flavonoids are polyphenolic compounds and have antioxidant properties that are not heat-resistant, therefore a suitable method is needed for extracting flavonoids, especially methods that do not use heat. Maceration extraction method is an extraction method by immersing simplicia at room temperature, while the Microwave Assisted Extraction (MAE) extraction method is an extraction method using microwave radiation. The study aims to determine the most effective method for flavonoid content of longan leaves using 2 extraction methods, namely maceration and MAE. Determination of flavonoid content was done using Aluminium chloride reagent whose absoption was measured using UV-Vis spectrophotometry. The results showed that the best extraction method to produce the highest flavonoid content in 70% ethanol extract of longan leaves was the Microwace Assisted Extraction method which produced flavonoid levels of 1.32 ± 0,09%.
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类黄酮乙醇提取物水平比较。采用不同的提取方法
龙眼植物(Dimocarpus logan L.)通常仅为其果实而被社区使用。龙眼叶含有次生代谢物,如类黄酮、多酚、皂苷和单宁。黄酮类化合物是一种多酚类化合物,具有抗氧化性,但不耐热,因此需要一种合适的提取方法,特别是不加热的方法。浸渍提取法是一种在室温下浸泡单纯物的提取方法,微波辅助提取法是一种利用微波辐射的提取方法。采用浸渍法和MAE两种提取方法,确定龙眼叶中黄酮类化合物含量的最有效提取方法。用氯化铝试剂测定黄酮类化合物的含量,紫外-可见分光光度法测定其吸光度。结果表明,70%乙醇龙眼叶中总黄酮含量最高的提取方法为微波辅助提取,总黄酮含量为1.32±0.09%。
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来源期刊
Ekologia Bratislava
Ekologia Bratislava Environmental Science-Ecology
CiteScore
1.80
自引率
0.00%
发文量
30
审稿时长
10 weeks
期刊介绍: The Journal Ecology (Bratislava) places the main emphasis on papers dealing with complex characteristics of ecosystems. Treated are not only general, theoretical and methodological but also particular practical problems of landscape preservation and planning. The ecological problems of the biosphere are divided into four topics: ecology of populations: study of plant and animal populations as basic components of ecosystems, ecosystem studies: structure, processes, dynamics and functioning of ecosystems and their mathematical modelling, landscape ecology: theoretical and methodical aspects, complex ecological investigation of territorial entities and ecological optimization of landscape utilization,
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