Biochemical characterization and Biolog based identification of efficient Jute retting bacterial isolates from retting water

S. S. Ray, Suparna Das, M. N. Ali, Lucina Yeas
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引用次数: 2

Abstract

Jute is considered as one of the cheapest natural fibre after cotton in terms of its production and uses. Retting is the most important post-harvest operation to yield high quality jute fibre and is solely carried out by various types of retting microorganisms. The present study was undertaken to screen and characterize the efficient retting microbes isolated from retting water based on their enzymatic activity followed by biolog based idenfication of those efficient microbes. These isolates were characterized on the basis of qualitative and quantitative estimation of Pectinolytic, Xylanase and Cellulase activity. Out of 40 isolated strains only 3 were finally identified as efficient jute retting microorganism having high pectinolytic and Xylanase activity coupled with less Cellulase activity. These identified three micro organisms may provide a suitable means to develop a new retting technique especially under water stress condition.
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黄麻絮凝水中高效分离菌的生化特性及生物学鉴定
在生产和使用方面,黄麻被认为是仅次于棉花的最便宜的天然纤维之一。干燥是获得高质量黄麻纤维最重要的收获后操作,完全由各种类型的干燥微生物进行。本研究是基于酶活性的基础上对从凝水中分离的高效凝水微生物进行筛选和表征,并对这些高效凝水微生物进行生物学鉴定。通过果胶分解酶、木聚糖酶和纤维素酶活性的定性和定量分析,对这些菌株进行了鉴定。在40株分离菌株中,只有3株被鉴定为高效的黄麻发酵微生物,具有较高的果胶分解酶和木聚糖酶活性,而纤维素酶活性较低。这三种微生物的鉴定可能为开发新的凝乳技术,特别是在水分胁迫条件下的凝乳技术提供了合适的手段。
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