Extracts of Ocimum gratissimum Leaves Inhibits Fe2+ and Sodium Nitroprusside Induced Oxidative Stress in Rat Liver

World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences Pub Date : 2015-05-25 DOI:10.7897/2277-4572.05318

O. Ojo, O. Oloyede

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引用次数: 2

Abstract

This study investigate the antioxidative properties and the ability of aqueous, ethanol and ethyl acetate extracts from Ocimum gratissimum (OG) leaves to inhibit some pro-oxidants (Fe and sodium nitroprusside) induced lipid peroxidation in rat’s liver homogenates in vitro. The ability of the extracts to inhibit 25 μM FeSO4 and 7.0 μM sodium nitroprusside induced lipid peroxidation in isolated rat’s liver was determined. The results of the study revealed that both pro-oxidants caused a significantly decrease in (p<0.05) accumulation of lipid peroxides. However, aqueous extract of Ocimum gratissimum shows a high ability to inhibit lipid production in the liver induced with SNP than Fe. Ethanol and ethyl acetate extract of Ocimum gratissimum which shows a high ability to inhibit lipid production more when induced with Fe than SNP. However, ethyl acetate fraction of Ocimum gratissimum shows a higher inhibitory effect on both Fe and SNP induced lipid peroxidation in rat’s liver. This might be as a result of its significantly higher extractable phytochemicals. Therefore, Fe II and sodium nitroprusside induced oxidative stress could be managed by dietary intake of Ocimum gratissimum leaves.

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茴香叶提取物对大鼠肝脏氧化应激的抑制作用

本研究考察了枸杞叶水提物、乙醇提物和乙酸乙酯提物对大鼠肝脏匀浆中一些促氧化剂(铁和硝普钠)诱导的脂质过氧化的抑制作用。测定提取物对25 μM FeSO4和7.0 μM硝普钠诱导的离体大鼠肝脏脂质过氧化的抑制能力。研究结果显示，两种促氧化剂均能显著降低脂质过氧化物的积累(p<0.05)。然而，与铁相比，茴香水提物对SNP诱导的肝脏脂质产生的抑制能力更强。铁诱导的茴香乙醇和乙酸乙酯提取物比SNP诱导的茴香提取物更能抑制油脂的产生。然而，竹叶乙酸乙酯部分对铁和SNP诱导的大鼠肝脏脂质过氧化均有较高的抑制作用。这可能是由于其可提取的植物化学物质明显更高。因此，铁离子和硝普钠诱导的氧化应激可以通过饲粮摄入西芹叶来调控。

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World Academy of Science, Engineering and Technology, International Journal of Pharmacological and Pharmaceutical Sciences

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