{"title":"Optimum culture conditions for the production of the extracellular pullulanase of Thermoactinomyces thalpophilus","authors":"F.J.C. Odibo , S.K.C. Obi","doi":"10.1016/0269-7483(90)90068-4","DOIUrl":null,"url":null,"abstract":"<div><p>The production of the thermostable pullulanase of <em>Thermoactinomyces thalpophilus</em> isolated from cow dung was studied in shake-flask cultures. Maximum production of pullulanase was obtained with 5% (w/v) soybean meal, 2% (w/v) yam starch, and Sr<sup>2+</sup>, at an initial culture pH of 5·0. The time-course of the synthesis of pullulanase indicated maximum production of the enzyme at 36 h, corresponding with the culture pH of 6·2.</p></div>","PeriodicalId":100177,"journal":{"name":"Biological Wastes","volume":"32 1","pages":"Pages 9-15"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0269-7483(90)90068-4","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Wastes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0269748390900684","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 3
Abstract
The production of the thermostable pullulanase of Thermoactinomyces thalpophilus isolated from cow dung was studied in shake-flask cultures. Maximum production of pullulanase was obtained with 5% (w/v) soybean meal, 2% (w/v) yam starch, and Sr2+, at an initial culture pH of 5·0. The time-course of the synthesis of pullulanase indicated maximum production of the enzyme at 36 h, corresponding with the culture pH of 6·2.
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