Optimum culture conditions for the production of the extracellular pullulanase of Thermoactinomyces thalpophilus

F.J.C. Odibo , S.K.C. Obi
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引用次数: 3

Abstract

The production of the thermostable pullulanase of Thermoactinomyces thalpophilus isolated from cow dung was studied in shake-flask cultures. Maximum production of pullulanase was obtained with 5% (w/v) soybean meal, 2% (w/v) yam starch, and Sr2+, at an initial culture pH of 5·0. The time-course of the synthesis of pullulanase indicated maximum production of the enzyme at 36 h, corresponding with the culture pH of 6·2.

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嗜thalophilus热放线菌胞外普鲁兰酶的最佳培养条件
研究了从牛粪中分离的嗜thalophilus热放线菌产耐热普鲁兰酶的摇瓶培养方法。在初始培养pH为5.0时,豆粕含量为5% (w/v),山药淀粉含量为2% (w/v), Sr2+含量为2%,普鲁兰酶产量最高。普鲁兰酶合成的时间过程表明,在培养pH为6·2时,36 h酶产量最大。
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