The Effect of Filteret Sugarcane Juice in Egg Yolk Citrate on Viability and Spermatozoa Abnormality, and pH of Bali Catle Semen

Rindiyani Y Tanii, A. A. Dethan, Theresia Ika Purwantiningsih
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Abstract

This study aims to determine the effect of the sugarcane juice diluent in egg yolk citrate on viability, spermatozoa abnormality, and pH of Bali bull semen. This study has been carried out in Poultry Housing of Animal Science Study Program, and the Laboratory of the Faculty of Agriculture, University of Timor. Semen is collected from a a bull, ± 4.5 years old, in healthy condition. The method used in this study was a completely randomized design method with 5 treatments and 4 replications. The treatment giving are: T0 (diluent citrate-egg yolk without sugarcane juice); T1 (diluent citrate-egg yolk + 5ml sugarcane juice); T2 (diluent citrate-egg yolk + 10 ml sugarcane juice); T3 (diluent citrate-egg yolk + 15 ml sugarcane juice) and T4 (diluent citrate-yolk + sugar cane juice 20 ml). The variables measured were viability, abnormal spermatozoa, and semen pH of Bali bull. The results showed that T4 treatment with a 20 ml sugarcane juice level showed the best value for spermatozoa viability (92.875%), spermatozoa abnormality (6.375%), and semen pH (6.6). Conclusion: that the level of 20 ml of sugarcane juice in semen diluent can maintain the life of Bali bull spermatozoa.
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柠檬酸蛋黄中过滤甘蔗汁对巴厘牛精液活力、精子异常及pH值的影响
本研究旨在研究蛋黄柠檬酸盐中甘蔗汁稀释剂对巴厘公牛精液活力、精子异常和pH值的影响。这项研究是在动物科学研究方案的家禽舍和帝汶大学农学院实验室进行的。精液采集自一头健康公牛,年龄±4.5岁。本研究采用完全随机设计方法,5个处理,4个重复。处理方法为:T0(柠檬酸-蛋黄稀释液,不加甘蔗汁);T1(柠檬酸稀释液-蛋黄+ 5ml甘蔗汁);T2(柠檬酸稀释液-蛋黄+ 10ml甘蔗汁);T3(柠檬酸盐蛋黄稀释液+ 15ml甘蔗汁)和T4(柠檬酸盐蛋黄稀释液+甘蔗汁20ml)。测量的变量为巴厘公牛的活力、异常精子和精液pH。结果表明,20 ml甘蔗汁水平的T4处理对精子活力(92.875%)、精子异常(6.375%)和精液pH(6.6)的影响最大。结论:精液稀释剂中甘蔗汁20 ml的水平可维持巴厘公牛精子的寿命。
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