Nuclear DNA content of ten rice species as determined by flow cytometry

C. Martínez, K. Arumuganathan, Harumi Kikuchi, E. Earle
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引用次数: 37

Abstract

Accurate estimations of nuclear DNA content of rice are important because this crop is being used in many types of molecular studies. The main objective of this study was to estimate the nuclear DNA content in ten rice species by flow cytometry. Most of the values obtained were lower than those obtained in earlier studies using Feulgen microdensitometry. Significant differences in genome size among rice species were found. Oryza glaberrima had the smallest genome (0.73-0.76 pg/2C), while O. minuta and O. latifolia had the largest values (ca. 2.33 pg/2C). Tetraploid species had more nuclear DNA than diploid ones. The AA genome had less DNA (0.86-0.96 pg/2C) than the CC (1.14-1.17 pg/2C) and EE (1.99 pg/2C) genomes. The AA genome of O. saliva was larger than the AgA g genome of O. glaberrima and the A1A 1 genome of O. longistaminata, which had mean values of 0.73-0.76 and 0.78 pg/2C, respectively. Indica (IR36) and japonica (Yukihikari) cultivars of O. saliva also showed significant differences. Three different nuclear DNA levels (1.93, 1.85, and 1.31 pg/2C) were found among five O. ridleyi plants analyzed. Flow cytometry also allowed rapid and reliable determination of the ploidy level of anther culture-derived plants.
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用流式细胞术测定10种水稻的核DNA含量
准确估计水稻的核DNA含量是很重要的,因为这种作物被用于许多类型的分子研究。本研究的主要目的是利用流式细胞术估计10个水稻品种的核DNA含量。所获得的大多数值低于早期使用Feulgen微密度测定法所获得的值。水稻品种间基因组大小存在显著差异。glaberrima的基因组最小(0.73 ~ 0.76 pg/2C),而O. minuta和O. latifolia的基因组值最大(约2.33 pg/2C)。四倍体物种比二倍体物种有更多的核DNA。AA基因组的DNA含量(0.86 ~ 0.96 pg/2C)低于CC (1.14 ~ 1.17 pg/2C)和EE (1.99 pg/2C)基因组。唾液O.的AA基因组比光斑O.的AgA g基因组大,其平均值分别为0.73 ~ 0.76 pg/2C和0.78 pg/2C。籼稻(IR36)和粳稻(Yukihikari)品种也存在显著差异。结果表明,5种植物的核DNA水平分别为1.93、1.85和1.31 pg/2C。流式细胞术还可以快速可靠地测定花药培养植物的倍性水平。
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