{"title":"Purification and Properties of Carboxypeptidase from Tilapia Intestine : Digestive Enzyme of Tilapia-VIII","authors":"A. Taniguchi, K. Takano","doi":"10.2331/SUISAN.67.1096","DOIUrl":null,"url":null,"abstract":"テラピア腸管からアミノペプチダーゼを抽出し,各種クロマトグラフィーにて精製し,アミノペプチダーゼIおよびIIを得た。IおよびIIの最適pHは7.0および6.5,最適温度は45℃および50℃であり,活性はそれぞれpH6.5～9.0,PH6.5～8.5および45℃,50℃以下で安定であった。両酵素ともに,EDTA,o-フェナントロリンによって阻害され,Zn2+,Co2+,Mg2+により活性の回復が認められ,金属酵素の性質を示した。IはAla-pNA,IIはLeu-pNAに良く作用した。","PeriodicalId":9361,"journal":{"name":"Bulletin of the Japanese Society of Scientific Fisheries","volume":"24 1","pages":"1096-1102"},"PeriodicalIF":0.0000,"publicationDate":"2001-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bulletin of the Japanese Society of Scientific Fisheries","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2331/SUISAN.67.1096","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
