Ryosuke Morimoto, Aya Sakagami, Takenori Nakagaki, Hidenobu Sumitani, Y. Isegawa
{"title":"Growth-inhibitory Components in the Juice of Aronia melanocarpa with Activity against Staphylococcus aureus","authors":"Ryosuke Morimoto, Aya Sakagami, Takenori Nakagaki, Hidenobu Sumitani, Y. Isegawa","doi":"10.4327/JSNFS.71.161","DOIUrl":null,"url":null,"abstract":"Summary : Staphylococcus aureus is a resident bacterium in healthy humans. However, methicillin-resistant S. aureus (MRSA) is a major causative bacterium of nosocomial infectious diseases. In addition, the enterotoxin produced by S. aureus causes food poisoning. In this study, we searched for components within the juice of aronia ( Aronia melanocarpa ) that would exert antibacterial activity against the growth of S. aureus . To search these active components, we carried out different extraction methods. We found that the water-soluble components of freeze-dried aronia juice had a strong antimicrobial effect against S. aureus strains. To search for the active in-gredients, we carried out fractionation of aronia juice by reverse-phase chromatography and examined the antibacterial effects of fractions eluted with 20-30 % acetonitrile. Mass spectrometry identified some low-molecular-weight compounds ― chlorogenic acid, protocatechuic acid, and gentisic acid ― as components of aronia.","PeriodicalId":19296,"journal":{"name":"Nippon Eiyo Shokuryo Gakkaishi","volume":"63 1","pages":"161-166"},"PeriodicalIF":0.0000,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nippon Eiyo Shokuryo Gakkaishi","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4327/JSNFS.71.161","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Summary : Staphylococcus aureus is a resident bacterium in healthy humans. However, methicillin-resistant S. aureus (MRSA) is a major causative bacterium of nosocomial infectious diseases. In addition, the enterotoxin produced by S. aureus causes food poisoning. In this study, we searched for components within the juice of aronia ( Aronia melanocarpa ) that would exert antibacterial activity against the growth of S. aureus . To search these active components, we carried out different extraction methods. We found that the water-soluble components of freeze-dried aronia juice had a strong antimicrobial effect against S. aureus strains. To search for the active in-gredients, we carried out fractionation of aronia juice by reverse-phase chromatography and examined the antibacterial effects of fractions eluted with 20-30 % acetonitrile. Mass spectrometry identified some low-molecular-weight compounds ― chlorogenic acid, protocatechuic acid, and gentisic acid ― as components of aronia.