Y. Cho, S. Lee, B. Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B.J. Kim
{"title":"High-Throughput Single-Cell Electroporation Microchip with Three Dimensional Si Microelectrodes for Gene Transfection","authors":"Y. Cho, S. Lee, B. Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B.J. Kim","doi":"10.1109/SENSOR.2007.4300286","DOIUrl":null,"url":null,"abstract":"We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.","PeriodicalId":23295,"journal":{"name":"TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference","volume":"1079 1","pages":"939-942"},"PeriodicalIF":0.0000,"publicationDate":"2007-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"TRANSDUCERS 2007 - 2007 International Solid-State Sensors, Actuators and Microsystems Conference","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/SENSOR.2007.4300286","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.