High-Throughput Single-Cell Electroporation Microchip with Three Dimensional Si Microelectrodes for Gene Transfection

Y. Cho, S. Lee, B. Le Pioufle, N. Takama, S. Takeuchi, T. Fujii, B.J. Kim
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引用次数: 1

Abstract

We present details on fabrication of electroporation microchip, practical experiments of single-cell electroporation with our fabricated microchip. Also, the continuous electroporation for the continuous flow of cells is used for high-throughput electroporation. The delivery efficiency and cell viability tests are provided and the successful GFP transfection into cells is also evaluated with a fluorescent microscope after electroporation.
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高通量单细胞电穿孔芯片与三维硅微电极基因转染
我们详细介绍了电穿孔微芯片的制作,以及用我们制作的微芯片进行单细胞电穿孔的实际实验。此外,连续电穿孔用于细胞的连续流动,用于高通量电穿孔。提供了传递效率和细胞活力测试,并在电穿孔后用荧光显微镜评估了GFP成功转染细胞。
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