Proteome analysis of silkworm 2. Hemolymph

H. Kajiwara, A. Imamaki, Masatoshi Nakamura, K. Mita, Q. Xia, M. Ishizaka
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引用次数: 10

Abstract

Proteomic analysis of silkworm hemolymph at the fifth-instar day-3 larva stage was performed by combination of two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. Prominent spots on 2D-gel were analyzed after trypsinization and proteins were identified by Mascot and Sequest using the amino acid sequence data obtained from the genome sequence of silkworm. The gel images were tracked from the fourth-instar day-1 larva stage to the stage before adult moth for twenty-one days. The results of mass spectrometry analysis and changes in gel images are summarized in the silkworm proteome database (http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi).
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家蚕蛋白质组学分析2。血淋巴
采用二维聚丙烯酰胺凝胶电泳和质谱联用技术对家蚕5龄第3天幼虫的血淋巴进行了蛋白质组学分析。利用家蚕基因组序列获得的氨基酸序列数据,对2D-gel上的突出斑点进行胰蛋白酶化分析,并通过Mascot和Sequest对蛋白质进行鉴定。凝胶图像从4龄第1天幼虫期追踪到成蛾前21天。质谱分析结果和凝胶图像变化汇总在蚕蛋白质组数据库(http://kaiko2ddb.dna.affrc.go.jp/cgi-bin/search_2DDB.cgi)中。
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