The Chloride Sensitivity of Dunaliella parva Enzymes

H. Gimmler, R. Kaaden, U. Kirchner, A. Weyand
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引用次数: 50

Abstract

The NaCl resistance of enzymes in crude extracts from the extreme halotolerant alga Dunaliella parva was investigated. Enzymes exhibit a very broad scale of sensitivity against NaCl, ranging from half maximal inhibitions at 100 mM up to the molar range. Salt resistance depends on the type of the enzyme as well as on the substrate concentrations: Enzymes with nonelectrolytes exhibit a relatively higher salt tolerance than those with anionic substrates. Salt — inactivation is caused by Cl- and not by K+ or Na+. A competitive type of interaction of the Cl- anion with the anionic substrate is observed. Consequently high concentrations of anionic substrates protect enzymes in vivo against Cl- inactivation.

Half maximal inhibition of enzymes from D. parva cells adapted to different salinities were not significantly different under substrate saturating conditions, but the KM-Values for the substrates increased with the salinity of the preculture.

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细小杜氏藻酶对氯化物的敏感性
研究了极端耐盐藻杜氏藻粗提物中酶的耐盐性。酶对NaCl表现出非常广泛的敏感性,从100 mM的最大抑制量的一半到摩尔范围。耐盐性取决于酶的类型以及底物浓度:含有非电解质的酶比含有阴离子底物的酶表现出相对更高的耐盐性。盐失活是由Cl-而不是由K+或Na+引起的。观察到Cl-阴离子与阴离子底物的竞争性相互作用。因此,高浓度的阴离子底物在体内保护酶免受Cl-失活。在底物饱和条件下,适应不同盐度的小孢子虫细胞对酶的半最大抑制没有显著差异,但底物的km -值随着预培养盐度的增加而增加。
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