Study of Protective Effects of Calendula Officinalis L. Hydrethanolic Extract on Blood Parameters in Wistar Rats Treated With Cyclophosphamide

Abstract

Background and Objectives: Some of drugs which used in chemotherapy have destructive effects on hemopoetic tissue. Cyclophosphamid is a drug for malignant cancers treatment with subversion effects on blood hemopoetic bone marrow. Medicinal plants with antioxidant effects have anti-inflammatory and protective effects on bone marrow tissue. The aim of this study is to investigate of protective effect of Calendula officinalis L. hydrethanolic extract (CHE) on boold parameters in Wistar male rats induced with cyclophosphamide. Methods: In this experimental study 35 male Wistar rats (200-220 g) were divided in 5 groups(n=7): control (0.5 ml salin normal), Cyclophosphamide (15 mg/kg), positive control 600 mg/kg, CHE), treatment 1 (Cyclophosphamide 15 mg/kg‌+300 mg/kg, CHE) and treatment 2 (Cyclophosphamide 15mg/kg +600 mg/kg, CHE). All injections were done interaperituneally and the experiments were done for 15 days. At the end of expriments all animals were anesthetized (ketamine hydrochlorids, 50mg/kg+xylasine hydrochloride 10 mg/kg) and blood samples and sternum bone tissue (fixed in 10% formaldehyde) were collected. All data were evaluated statistically by ANOVA and Tukey’s method. Results: The results of this study were showed that cyclophosphamide has reduce effects on blood parameters (RBC, WBC, PL, Hb, Hct, MCV,MCH, MCHC) compare with control group significantly. CHE can protect and inhibite of cyclophosphamide damage effects on blood hemopoetic tissue. Conclusion: The use of CHE is able to protect of blood hemopoetic tissue in bone marrow and prohihite of cyclophosphamide effects.