Green Biosynthesis of Silver Nanoparticles Using Musa Acuminata Aqueous Flower Extract and Its Anti-Microbial Activities

O. Ogunmodede, J. Johnson, R. Osunlana, S. Olarenwaju, H. Ndu-Okeke
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Abstract

Nanomaterials have gained much relevant in the world of science due to their applications in catalysis, wastewater treatment and desulfurization of fossil fuels, biotechnology, pharmaceuticals and medicine. The green approach of nanoparticle synthesis employs the use of non-toxic reagents and is now preferred to the other methods which include thermal decomposition, electrochemical, photochemical, microwave assisted process chemical methods. Silver nanoparticle was biosynthesized using flower extract of Musa acuminata as reducing and capping agents. The synthesized silver nanoparticle was confirmed by the colour change after addition of the flower extract of Musa acuminata into silver nitrate solution. The silver nanoparticle was characterized by UV–Visible spectrophotometer, scanning electron microscopy (SEM), energy-dispersive x-ray spectroscopy (EDX), and Fourier transform infrared (FTIR) spectrophotometer. The result of SEM reveals the formation of silver nanoparticle which was spherical in shape with varying sizes ranged between 20-30 nm. The biosynthesized silver nanoparticle gave absorption at 375 nm, revealed silver metal as the most abundant element, vibrational bands indicating the presence of quinone, amides and conjugated ketone which served as reducing and capping agent. The bio-synthesized silver nanoparticles revealed potent antibacterial activity and the economical synthesis of silver nanoparticle from aqueous flower extract of Musa acuminata which is ecofriendly. Keywords :Green synthesis; silver nanoparticles; flower extract; capping agents; antibacterial activity; scanning electron microscopy. DOI : 10.7176/CMR/11-7-02 Publication date :September 30 th 2019
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麝香花提取物绿色合成纳米银及其抑菌活性研究
由于纳米材料在催化、废水处理和化石燃料脱硫、生物技术、制药和医学等领域的应用,纳米材料已经在科学界得到了广泛的应用。纳米颗粒合成的绿色方法使用无毒试剂,现在比热分解、电化学、光化学、微波辅助工艺化学等方法更受欢迎。以麝香花提取物为还原剂和封盖剂,生物合成纳米银颗粒。将荆芥花提取物加入硝酸银溶液后,通过其颜色变化来证实所合成的纳米银。采用紫外可见分光光度计、扫描电镜(SEM)、能量色散x射线能谱仪(EDX)和傅里叶变换红外分光光度计(FTIR)对纳米银进行了表征。扫描电镜结果显示,纳米银颗粒呈球形,粒径在20 ~ 30 nm之间。生物合成的银纳米粒子在375 nm处吸收,显示金属银是最丰富的元素,振动带表明醌、酰胺和共轭酮的存在,它们起到还原和封盖作用。生物合成的银纳米粒子显示出了强大的抗菌活性,并证明了以麝香花水提物为原料合成银纳米粒子的经济性和环保性。关键词:绿色合成;银纳米粒子;花中提取;限制代理人;抗菌活性;扫描电子显微镜。出版日期:2019年9月30日
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