Dextransucrase immobilized on activated-chitosan particles as a novel biocatalyst

Q2 Chemical Engineering Journal of Molecular Catalysis B-enzymatic Pub Date : 2016-11-01 DOI:10.1016/j.molcatb.2016.12.007

Natália G. Graebin, Diandra de Andrades, Marina C. Bonin, Rafael C. Rodrigues, Marco A.Z. Ayub

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引用次数: 10

Abstract

Dextransucrase from Leuconostoc mesenteroides B-512F was covalently immobilized on glutaraldehyde-actived chitosan particles. The best initial protein loading (400 mg/g of dried support) showed 197 U/g of catalytic activity. The optimal reaction pH and temperature of this new biocatalyst were determined to be 4.5 and 20 °C, respectively. Regarding the thermal stability, the immobilization enhanced enzyme protection against high temperatures, whereas glucose and maltose acted as stabilizers. The biocatalyst was stable under storage at 5 °C for a month. The biocatalyst presented good operational stability, retaining up to 40% of its initial activity after ten batch cycles of reaction to obtain oligosaccharides. These results suggest the use of the immobilized dextransucrase on chitosan particles as a promising novel biocatalyst to produce dextran and oligosaccharides.

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固定化葡聚糖酶作为一种新型生物催化剂

以戊二醛活化的壳聚糖颗粒为载体，共价固定了肠系膜白菌B-512F葡聚糖酶。最佳初始蛋白质负荷(400mg /g)的催化活性为197u /g。该新型生物催化剂的最佳反应pH为4.5℃，反应温度为20℃。热稳定性方面，固定化酶增强了酶对高温的保护作用，而葡萄糖和麦芽糖则起到了稳定作用。该生物催化剂在5℃条件下稳定保存一个月。该生物催化剂表现出良好的操作稳定性，经过10批循环反应获得低聚糖后，其初始活性保持在40%以上。这些结果表明，在壳聚糖颗粒上固定化右旋糖酐酶是一种很有前途的新型生物催化剂，可用于生产右旋糖酐和低聚糖。

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来源期刊

Journal of Molecular Catalysis B-enzymatic 生物-生化与分子生物学

CiteScore

2.58

自引率

0.00%

发文量

审稿时长

3.4 months

期刊介绍： Journal of Molecular Catalysis B: Enzymatic is an international forum for researchers and product developers in the applications of whole-cell and cell-free enzymes as catalysts in organic synthesis. Emphasis is on mechanistic and synthetic aspects of the biocatalytic transformation. Papers should report novel and significant advances in one or more of the following topics; Applied and fundamental studies of enzymes used for biocatalysis; Industrial applications of enzymatic processes, e.g. in fine chemical synthesis; Chemo-, regio- and enantioselective transformations; Screening for biocatalysts; Integration of biocatalytic and chemical steps in organic syntheses; Novel biocatalysts, e.g. enzymes from extremophiles and catalytic antibodies; Enzyme immobilization and stabilization, particularly in non-conventional media; Bioprocess engineering aspects, e.g. membrane bioreactors; Improvement of catalytic performance of enzymes, e.g. by protein engineering or chemical modification; Structural studies, including computer simulation, relating to substrate specificity and reaction selectivity; Biomimetic studies related to enzymatic transformations.