Screening of polychlorinated biphenyls in insulating oil using a microfluidic based pretreatment and immunoassay

Arata Aota , Yasumoto Date , Shingo Terakado , Naoya Ohmura
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引用次数: 3

Abstract

Polychlorinated biphenyls (PCBs) are persistent organic pollutants in insulating oil of a large number of transformers. A rapid and economical analytical method to detect PCB contamination is still required. To address this issue, we propose here the first microfluidic screening method for PCB contamination in insulating oil. The insulating oil was pretreated using a multilayer capillary column and a microfluidic liquid–liquid partitioning. PCBs in the pretreated oil were measured using a microfluidic kinetic exclusion assay. In order to detect PCBs with high sensitivity, conditions of the microfluidic kinetic exclusion assay were optimized. Measurements were rapidly completed (within 43 min). The measurement range was estimated to be 0.26–3.3 mg/kg defined as the relative absorbance from 20% to 80%. The screening performance (false positive and false negative rates) was tested on fifty real oil samples; results about these tests were discussed in detail, especially suitable cutoff by comparing with the data analyzed using high-resolution-gas-chromatography/high-resolution-mass-spectrometry. Finally, the screening performance was confirmed using our proposed stochastic screening model. A cutoff of 0.3 to judge as positive is suitable considering the risk of the PCB release into the environment.

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用微流体预处理和免疫分析法筛选绝缘油中的多氯联苯
多氯联苯(PCBs)是大量变压器绝缘油中的持久性有机污染物。目前仍需要一种快速、经济的分析方法来检测PCB污染。针对这一问题,本文首次提出了绝缘油中PCB污染的微流控筛选方法。采用多层毛细管柱和微流控液液分流对绝缘油进行预处理。采用微流体动力学排除法测定预处理油中的多氯联苯。为提高检测多氯联苯的灵敏度,对微流控动力学排除法的条件进行了优化。测量迅速完成(43分钟内)。测量范围为0.26 ~ 3.3 mg/kg,相对吸光度为20% ~ 80%。在50个实际油样上测试了筛选性能(假阳性和假阴性率);通过与高分辨率气相色谱/高分辨率质谱分析数据的比较,详细讨论了这些测试的结果,特别是适合的截止。最后,利用我们提出的随机筛选模型对筛选性能进行了验证。考虑到PCB释放到环境中的风险,0.3的截止值判断为正值是合适的。
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