Comparison of impact of various application methods for the blood plasm, enriched by the thrombocytes, on healing of the burn and scalped wounds in experiment

Abstract

Objective. Comparison, in conditions of experiment between impact of the blood plasm, enriched by thrombocytes, on the terms of the burn and scalped wounds healing and morphological characteristics of the cicatrices formatted in its injectional or noninjectional introduction, using the method of electrophoresis. Materials and methods. The investigations were conducted on 30 white rats, which were divided into three groups with 10 animals in every group, median body mass of the animals have constituted (275.5 ± 15.1) gr. After achievement of medicinal sleep (intraperitoneal introduction of 1.0 ml of 0.5% solution of sodium thiopental in combination with 0.2 ml of propofol 1% solution the scalped skin incisions with the 1.5 × 0.5 cm dimensions were performed to the animals. Besides that, one of the incisions was processed with diathermy up to the scab crust formation. Both wounds were left open, without sutures. To the control group animals after the wounding conduction no procedures were performed, the wounds have been healed in primary pattern. To the Group I animals during two consequent weeks the blood plasm, enriched by thrombocytes, was introduced, using intracutaneous injections twice a week in quantity of 10 ml, puncturing the injured zone around. To the investigation Group II animals the electrophoresis in accordance to own procedure through napkin, soaked in the blood plasm, enriched by thrombocytes, was performed with the same rate. The blood plasm, enriched by thrombocytes, was prepared from the whole venous blood of human Group A(0), using centrifugation for achievement of targeted quantity of thrombocytes 850 000 in 1 ml. Morphological features of cicatrices formatted and their width were studied, using the methods of light microscopy and morphometry on the 21th day of experiment. Results. Application of the blood plasm, enriched by thrombocytes, was accompanied by statistically significant reduction of the cicatrices width, which were formatted on the 21-th day in locations of the scalped wounds simulation (p < 0.05 and p < 0.01 accordingly, for injectional and electrophoretic methods of application in comparison with the control group) and the burn (p < 0.01 and p < 0.005, accordingly, for the injectional and electrophoretic methods of application in comparison to the control group) wound. At the same time the cicatrices width in electrophoretic application of the blood plasm, enriched by thrombocytes, was statistically significantly lesser (p < 0.05), than in its injectional introduction, not depending on the wound type. Besides that, the cicatrices connective tissue while application of the blood plasm, enriched by thrombocytes, in a moment of the animals evacuation from the experiment have looked like more mature and differed by enhanced organization of the collagen fascicles localization. Conclusion. The method of noninjectional introduction of the blood plasm, enriched by thrombocytes, which was proposed, owes certain advantages over its standard injectional application, which constitute the enhanced degree of maturation of connective tissue, more organized localization of the collagen fascicles, lesser width of the cicatrices evolved and the procedure painlessness. Noninjectional introduction of the blood plasm, enriched by thrombocytes, using method of electrophoresis is free from postinjectional complications, typical for standard methods of its introduction.