Impact of Gibberellic Acid and Organic Growth Media on Seed Germination and Seedling Development of Rubber (Hevea brasiliensis)

Ganih Indin Sappalani, L. M. Cabahug, Vences C. Valleser
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引用次数: 1

Abstract

One of the best interventions to hasten seed germination and seedling development is the exogenous application of phytohormone. This study evaluated the germination and seedling development of rubber seeds in response to gibberellic acid (GA3) and organic growth media under nursery condition. The experiment was arranged in a 4x3 factorial experiment in completely randomized design (CRD) with three replications. Growth media (including: top soil, TS; TS + rice hull, RH; TS + chicken dung, CD; TS + vermicast, VC) were considered as Factor A, and different concentrations of GA3 (0, 50, and 100 ppm) were comprised as the Factor B. Newly fallen seeds (with no defects) from existing ‘RRIM 600’ rubber tree plantation were collected and used in the experiment. Seeds were subjected to float in GA3 solutions based on corresponding treatments for 18 h prior to sowing in different organic growth media. GA3 (100 ppm) led to the earliest emergence, highest germination percentage and normal seedlings with least mortality. The TS+VC was the best growth media influencing germination. The TS+VC media regardless of the concentration of GA3 caused 100% normal seedlings. Likewise, the combination of TS+CD as growth media and 100 ppm of GA3 also produced promising percentage of normal seedlings of rubber with 97.33%. Seedling mortality was lessen using the combination of TS+VC as growth media and 100 ppm GA3. Generally, application of 100 ppm GA3 and top soil mixed with vermicast as growth media provoked better seed germination and early seedling development in rubber.
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赤霉素和有机培养基对橡胶树种子萌发和幼苗发育的影响
促进种子萌发和幼苗发育的最佳干预措施之一是外源施用植物激素。在苗圃条件下,研究了赤霉素(GA3)和有机培养基对橡胶种子萌发和幼苗发育的影响。试验采用完全随机设计(CRD), 4 × 3阶乘试验,3个重复。生长介质(包括:表土、TS;TS +稻壳,RH;TS +鸡粪,CD;以TS + vermicast, VC)为因子A,以不同浓度的GA3(0、50和100 ppm)为因子b。从现有的“RRIM 600”橡胶林中收集新落种子(无缺陷)用于实验。种子在不同有机生长介质中播种前,按相应处理在GA3溶液中浮沉18h。GA3 (100 ppm)出苗最早,发芽率最高,幼苗正常,死亡率最低。TS+VC是影响萌发的最佳培养基。在TS+VC培养基中,无论GA3浓度如何,均可产生100%正常幼苗。同样,TS+CD为生长介质,GA3浓度为100 ppm,也能产生97.33%的橡胶正常苗。以TS+VC和100 ppm GA3组合作为生长介质,可降低幼苗死亡率。一般来说,施用100 ppm GA3和表土混合蚯蚓浆作为生长介质,橡胶种子萌发和幼苗早期发育较好。
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