Effect of Monoclonal Antibody to Human Zona Pellucida 3 in Luteineizing Hormone Receptor Expression, Estradiol Levels and de Graaf Follicles Quantity in Mice

Abstract

Objective: This research aimed to evaluate effect of monoclonal antibody to human Zona Pellucida 3 (Mab-hZP3) on luteineizing hormone (LH) receptor expression, estradiol levels and de Graaf follicles quantity of mus musculus mice ovaries. Materials and Methods: True experiment post test only control group design was used as research method. Treatments used were control (adjuvant in Tris HCl), and Mab-hZP3 with dosages of 20 μg, 40 μg and 60 μg which tested on day 10, 15 and 20. Luteineizing hormone (LH) receptor, estradiol level and the number of de Graaf follicles measurement were done by immunohistochemical test, ELISA method, and hematoxilin eosin staining, respectively. Results: The results showed that there was no significant difference found on Mab-hZP3 interaction between 20 μg-60 μg dosages and 10–20 days of observation time towards LH receptor expression, estradiol level and and the number of de Graaf follicles. This was related to specificity of monoclonal antibody used. Conclusion: Mab-hZP3 did not lower LH receptor expression, estradiol level and the number of de Graaf follicles quantity, which means that Mab-hZP3 did not interfere at folliculogenesis and not altering hormones profile. It can be concluded that Mab-hZP3 has the potential to be a safe immnocontraception material.