Allelophatic and Antioxidant Activity of Eight Amazon Species from De Tamshiyacu Tahuayo Reserve

Q4 Agricultural and Biological Sciences Polish Journal of Natural Sciences Pub Date : 2019-01-01 DOI:10.15640/jns.v7n1a2
Úrsula Monteiro, B. Díaz, R. Saldaña, V. Sotero, D. García
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Abstract

In this study were evaluated the antioxidant activity (AAO), allelopathic (ALL) and bioactive substances present in leaves of eight plant species from the Tamshiyacu-Tahuayo Community (Loreto-Peru). The AAO evaluation was performed by the free radical DPPH capture method, using dry and pulverized leaves, macerated in methanol for fifteen days, solutions of 0.015.0 mg/mL and 0.1 mM DPPH were prepared, reading was performed in the UV/vis spectrometer at 517 nm, for the ALL was performed by the Petri plates method, using methanolics extracts of fresh leaves with concentrations of 0.150.0 mg/mL against the growth of pregerminated seeds of Latuca sativa , the reading was made by measuring the hypocotyl and radicle of the seedling. In the results of species with high AAO were Virola sebifera, Caryocar glabrum and Tapirira guianensis, which present a percentage of inhibition superior to 50% in concentrations of 5.0 mg/mL. The species with high ALL are Xylopia benthami, Malouetia naias and Virola surinamensis, reaching an EC50 for the radicle at concentrations lower than 1.0 50.0 mg/L. The concentration of phenolic compounds for these species varied from 115217,18-321815,14 mg/kg, flavonoids from 1030,79-1441,16 mg/kg and proanthocyanidins from 0.65-1,77 mg / 100g. Total alkaloids range from 0,0 68,2 m/g evaluation, presenting differences between them.
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塔华约保护区8种亚马逊树植物化感及抗氧化活性研究
本研究对秘鲁Tamshiyacu-Tahuayo群落8种植物叶片的抗氧化活性(AAO)、化感作用(ALL)和生物活性物质进行了研究。AAO评价采用自由基DPPH捕获法,用干燥粉碎的叶片,在甲醇中浸泡15天,配制0.015.0 mg/mL和0.1 mM DPPH溶液,在517 nm紫外/可见光谱仪上进行读取,对于ALL采用培养皿法,使用浓度为0.150.0 mg/mL的新鲜叶片甲醇提取物对Latuca sativa预萌发种子的生长进行测定。通过测量幼苗的下胚轴和胚根来获得读数。结果表明,AAO高的菌种为紫罗拉(Virola sebifera)、山核桃(Caryocar glabrum)和贵州貘(Tapirira guianensis),在5.0 mg/mL浓度下,其抑制率超过50%。ALL较高的物种为底栖木藻(Xylopia benthami)、马洛埃提亚(Malouetia naias)和苏里南毒蛾(Virola surinamensis),浓度低于1.0 ~ 50.0 mg/L时,其根的EC50值达到最高。酚类化合物含量为115217、18 ~ 321815、14 mg/kg,黄酮含量为1030、79 ~ 1441、16 mg/kg,原花青素含量为0.65 ~ 1、77 mg/ 100g。总生物碱的评价范围为0、0、68、2 m/g,存在差异。
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来源期刊
Polish Journal of Natural Sciences
Polish Journal of Natural Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
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0.60
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