Association of Exon 1 Variants of NOEY2 Gene with Poly cystic Ovary Syndrome (PCOS) and Diabetes

Abstract

Methods: This study was conducted on 240 blood samples (1 each) from women in four groups: polycystic, diabetes, polycystic-diabetes, and control. Genomic DNA extraction was performed using the extraction kit, according to the protocol. A primer pair was designed and synthesized for the exon 1 sequence of the NOEY2 gene. After gene amplification by polymerase chain reaction (PCR) and evaluation of band quality, Sanger sequencing was performed on PCR products. Sequencing results were analyzed using Chromas, GeneRunner, and Alignment software, and statistical calculations were performed by SNPAlyze, SPSS, and Web-Assotest program. two and of nucleotides and in the region of a between affect the affinity to bind in certain transcription factors by allelic changes due to its location in the CTCF overlapping promoter region.