In vivo study of pH Dependent and Enzymatically Triggered Colon Targeted Tinidazole Microspheres

Abstract

Introduction: Shellac coated Tinidazole (TNZ) loaded pectin microspheres with the potential for colon targeted delivery has been evaluated to study in-vivo behavior. Materials and Methods: Biodistribution and pharmacokinetic studies were performed for the determination of concentration of TNZ in rats. This method was used to evaluate the colon targeting property of TNZ loaded microspheres. Pure TNZ or TNZ microspheres were given to rats by oral administration. Plasma and the different parts of gastrointestinal (GI) tract were taken after 2, 8, 12 and 24 h of oral administration of pure TNZ or TNZ microspheres to rats and the concentration of TNZ was measured. Results and Conclusion: Results obtained shows that pure TNZ distributes mainly in stomach and in low concentration to small intestine and colon. However, TNZ released from microspheres mainly distributes in colon. Therefore, this approach suggests that shellac coated TNZ loaded pectin microspheres has a good colon targeting property. Key words: Biodistribution study, Colon targeting, Pharmacokinetic study, Pectin microspheres, Tinidazole.