Effect of electric field on F-actin oriented by flow

Shoyu Kobayashi
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引用次数: 17

Abstract

Pulsed electric fields were applied to F-actin oriented by flow and the process of dis- orientation was followed by birefringence measurements. An electric field parallel to the filament axis of F-actin produced a large disorientation effect whereas a perpendicular field produced a small effect. The analysis of the birefringence decrease in normal and reversed pulses at various field strengths suggests that F-actin has a large permanent dipole moment perpendicular to the filament axis. The marked disorientation effect of the parallel field on F-actin vanished on addition of H-meromyosin. On dissociation of the complex of F-actin and H-meromyosin by the addition of ATP the disorientation effect of the parallel field was again observed. This result suggests that a strong electric interaction exists between (permanent) dipoles of F-actin and H-meromyosin.

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电场对流动取向f -肌动蛋白的影响
脉冲电场作用于流动取向的f -肌动蛋白上,然后进行双折射测量。平行于f -肌动蛋白丝轴的电场产生较大的定向失向效应,而垂直的电场产生较小的定向失向效应。对不同场强下正常和反向脉冲双折射率下降的分析表明,f -肌动蛋白具有垂直于丝轴的大的永久偶极矩。加入h -肌球蛋白后,平行场对f -肌动蛋白明显的定向障碍作用消失。在加入ATP解离f -肌动蛋白和h -肌球蛋白复合体时,再次观察到平行场的定向失向效应。这一结果表明f -肌动蛋白和h -肌凝蛋白(永久)偶极子之间存在强电相互作用。
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