{"title":"Effect of electric field on F-actin oriented by flow","authors":"Shoyu Kobayashi","doi":"10.1016/0926-6577(64)90097-X","DOIUrl":null,"url":null,"abstract":"<div><p>Pulsed electric fields were applied to F-actin oriented by flow and the process of dis- orientation was followed by birefringence measurements. An electric field parallel to the filament axis of F-actin produced a large disorientation effect whereas a perpendicular field produced a small effect. The analysis of the birefringence decrease in normal and reversed pulses at various field strengths suggests that F-actin has a large permanent dipole moment perpendicular to the filament axis. The marked disorientation effect of the parallel field on F-actin vanished on addition of H-meromyosin. On dissociation of the complex of F-actin and H-meromyosin by the addition of ATP the disorientation effect of the parallel field was again observed. This result suggests that a strong electric interaction exists between (permanent) dipoles of F-actin and H-meromyosin.</p></div>","PeriodicalId":100169,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1964-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6577(64)90097-X","citationCount":"17","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Specialized Section on Biophysical Subjects","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/092665776490097X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 17
Abstract
Pulsed electric fields were applied to F-actin oriented by flow and the process of dis- orientation was followed by birefringence measurements. An electric field parallel to the filament axis of F-actin produced a large disorientation effect whereas a perpendicular field produced a small effect. The analysis of the birefringence decrease in normal and reversed pulses at various field strengths suggests that F-actin has a large permanent dipole moment perpendicular to the filament axis. The marked disorientation effect of the parallel field on F-actin vanished on addition of H-meromyosin. On dissociation of the complex of F-actin and H-meromyosin by the addition of ATP the disorientation effect of the parallel field was again observed. This result suggests that a strong electric interaction exists between (permanent) dipoles of F-actin and H-meromyosin.