Compatibility, Phosphate Solubility and Phosphatase Activity by Phosphate Solubilizing Bacteria

Abstract

The phosphate solubilizing bacteria are soil microbe can dissolve P which is not available to become available to plants. They have capable to produce extracelluler enzyme, i.e. group of phosphatase enzyme which able to mineralized of organic P to inorganic P. The objective of this experiment was to examine compatibility test, phosphatase enzyme activity and phosphate solubility by phosphate solubilizing bacteria. The compatibility test was conducted qualitatively with the streak method. The design for bioassay of phosphate solubilizing phosphate for phosphatase activity test and P solubility was a randomized block design with five treatments and five replications. The phosphate solubilizing bacteria used were Burkolderia sp., Pseudomonas mallei, Bacillus substilis, and Bacillus megatherium. The results of the compatibility test on the phosphate solubilizing bacterial isolates showed that each isolate was compatible. Furthermore, the result of experiment revealed that phosphate solubilizing bacteria could produce phosphatase enzyme ranges between 4,53-4,82 μg pNP/g/h and P dissolved about 3909,55-6052,19 ppm. These phosphate solubilizing bacteria can be used as biofertilizers to increase soil P solubility.