Antimicrobial Activity of Barbatimão Glycol Extract on Streptococcus mutans

Abstract

The study of medicinal plants with antimicrobial action is a current issue, mainly due to the increase of bacterial resistance and the need for new antimicrobials with equal or greater efficacy, low cost and low side effects. The constant search for new products with bactericidal potential on oral microorganisms, mainly of natural origin, has grown considerably in recent years.The objective of this research was to evaluate the efficiency of concentrations of Barbatimao glycolic extract solution (Stryphnodendronadstringens) in inhibiting the bacteria growth associated with  in vitro dental caries. Sixteen different concentrations of Barbatimao glycolic extract were prepared  (0%, 1.56%, 3.12%, 4.68%, 6.25%, 7.81%, 9.37%, 10.93, 12, 5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, 100%) and the 2% chlorhexidine solution were  used as the control group. The prepared solutions were tested on the bacterial strain of Streptococcus mutans. Bacterial growth was observed on plates containing BHI culture medium. Barbatimao concentrates were placed in wells made in the culture medium and each plate was incubated in a CO2 oven (37 ° C / 24 hours). The test was performed in triplicate, resulting in the absence of bacterial inhibition at the tested barbatimao concentrations, with bacterial inhibition of 2% chlorhexidine being positive. It is concluded that the barbatimao glycolic extract in the  analyzed concentrations does not present bactericidal action on Streptococcus mutans. Keywords: Dental Caries. Microbiota. Stryphnodendronbarbatimam Resumo O estudo de plantas medicinais com acao antimicrobiana e um assunto atual, principalmente devido ao aumento da resistencia bacteriana e a necessidade de novos antimicrobianos com igual ou maior eficacia, baixo custo e baixos efeitos colaterais. A busca constante de novos produtos com potencial bactericida sobre os micro-organismos orais, principalmente de origem natural tem crescido bastante nos ultimos anos. O objetivo desta pesquisa foi avaliar a eficiencia de diferentes concentracoes de solucao de extrato glicolico de Barbatimao (Stryphnodendron adstringens) na inibicao do crescimento de bacterias associadas a carie dental in vitro.Foram preparadas dezesseis concentracoes do extrato glicolico do Barbatimao (0%, 1,56%, 3,12%, 4,68%, 6,25%, 7,81%, 9,37%, 10,93, 12,5%, 25%, 37,5%, 50%, 62,5%, 75%, 87,5%, 100%) e como grupo controle foi utilizada a solucao de clorexidina 2%. As solucoes preparadas foram testadas sobre a cepa bacteriana de Streptococcus mutans. O crescimento bacteriano foi observado em placas contendo meio de cultura BHI. As concentracoes do barbatimao foram colocadas em pocos realizados no meio de cultura e cada placa foi incubada em estufa de CO2 (37°C / 24 horas). O teste foi realizado em triplicata, tendo como resultado a ausencia de inibicao bacteriana para as concentracoes do barbatimao testadas, sendo positiva a inibicao bacteriana para clorexidina a 2%. Conclui-se que o extrato glicolico de barbatimao nas concentracoes analisadas, nao apresenta acao bactericida sobre o Streptococcus mutans. Palavras-chave: Carie Dentaria. Microbiota. Barbatimao