Sifat Antimikroba dan Pengaruh Perlakuan Bahan Baku terhadap Rendemen Minyak Sereh Wangi (Antimicrobial Properties and Effects of Raw Material Treatments on Citronella Oil Yield)

Pub Date : 2020-09-28 DOI:10.23960/JSL38411-419

Shintawati Shintawati, Oktaf Rina, D. Ermaya

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引用次数: 1

Abstract

Citronella oil is one of the non-timber forest products (NTFP) and commercially obtained from the distillation of the leaves of the Cymbopogon nardus L. This study aims to determine the effect of the size of the distillation raw material and storage time of the raw material on yield and determine the antimicrobial properties of citronella oil from plants in the production forest Register 40 KPH Ged o ng Wani, Lampung Selatan Regency. The research was conducted using a complete randomized block design (RAKL) with two factors, namely the size of the distillation raw material (whole leaf , 5 cm, 10 cm , and 15 cm) and the storage time of the raw material (fresh, 2 days , and 4 days) which was carried out in 3 replications. The variables measured were yield, specific gravity, and solubility of citronella oil in alcohol. The inhibition ability test of citronella oil against bacteria using essential oil obtained from distillation at the highest yield conditions, namely fresh leaves and leaf size of 5 cm using the diffusion well method. The results showed that the storage time factor, the size of the distillation raw material, and the interaction between the storage time and the size of the raw material had a significant effect on the yield of citronella oil. The highest yield of citronella oil was achieved in the condition of fresh leaves with a size of 5 cm, namely 2 , 09%. Density and solubility in alcohol of citronella oil produced met SNI 06-3953-1995 standard , respectively 0 , 8718-0 , 8928 g/ ml and 1:2. Citronella oil has strong antibacterial properties against Propioni acne, Escherichia coli, Staphylococcus aureus, and Bacillus cereus with inhibition zone diameters we re 40 , 2 0 mm , 18 , 36 mm , 13 , 07 mm, and 18 , 8 0 mm, respectively. Citronella oil from plants in Register 40 KPH Gedong Wani has potential as a raw material for the cosmetic and disinfectant industry. Keywords: antimicrobial, citronella oil, yield

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香茅油是一种非木材林产品(NTFP)，由香茅(Cymbopogon nardus L.)的叶子蒸馏得到。本研究旨在确定蒸馏原料的大小和原料的储存时间对产量的影响，并确定从生产森林中提取的香茅油的抗菌性能。采用完全随机区组设计(RAKL)，选取蒸馏原料尺寸(全叶，5 cm, 10 cm, 15 cm)和原料储存时间(新鲜，2 d, 4 d) 2个因素，进行3个重复。测定的变量为香茅油的收率、比重和醇溶度。采用扩散孔法在最高产率条件下，即鲜叶和叶片大小为5 cm时，用蒸馏得到的精油对香茅油的抑菌能力进行试验。结果表明，储存时间、蒸馏原料粒度以及储存时间与原料粒度的交互作用对香茅油的得率有显著影响。香茅油在5 cm的鲜叶条件下得率最高，为2.09%。所得香茅油的密度和醇溶度分别满足SNI 06-3953-1995标准，分别为0、8718-0、8928 g/ ml和1:2。香茅油对丙痘、大肠杆菌、金黄色葡萄球菌和蜡样芽孢杆菌具有较强的抑菌作用，抑菌圈直径分别为40、20 mm、18、36 mm、13、07 mm和18、80 mm。格东瓦尼地区的香茅精油有潜力作为化妆品和消毒剂行业的原料。关键词:抗菌;香茅油;收率

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