The effect of aerobic training and consumption of L-carnitine supplements on Gen expression of HMG-CoA reductase and LDL receptor in the liver of male Wistar rats Injected by Boldenone

Abstract

The aim of this study was to investigate the effect of aerobic training and consumption of L-carnitine supplements on HMG-CoA reductase and LDL receptor in the liver of male Wistar rats toxicated by Boldenone. 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, sham, boldenone (5mg/kg), L-carnitine, aerobic training- L-carnitine.The endurance moderate intensity training program (55-50% of maximal oxygen consumption) performed for 6 weeks and 5 times a week. Injection once a week, on an appointed day, and in the quadriceps and hamstring was conducted in depth. After anesthesia, autopsy was performed and the testes Isolated. The HMG-CoA reductase and LDL receptor expression in the samples was measured by Real Time PCR and the quantification of gene expression levels using the formula 2-ΔΔct were analyzed by One-way ANOVA and post hoc Scheffe at the significant level P<0. 05.  The results showed that aerobic training and supplementation with L-carnitine was significant effect on HMG CoA reductase and LDL-R in the liver of male Wistar rats toxicated by boldenone (P=0/000). The results showed that the expression of HMG-CoA reductase changes in the liver of male Wistar rats in Group training - L-carnitine significantly lower than the control group (P=0/000). Changes in the expression of LDL-R in groups training - L-carnitine and L-carnitine increased significantly compared to control group (P=0/000). According to the findings, it seems to supplementation with L-carnitine with regular aerobic training modulating the biosynthesis of cholesterol in liver tissue.