Label-Free, Microfluidic Separation of Human Breast Carcinoma and Epithelial Cells by Adhesion Difference

Abstract

A simple, label-free microfluidic separation of cancer cells by exploiting difference in cell adhesion. To maximize the adhesion difference, three types of polymeric nanostructures (50 nm pillars, 50 nm perpendicular and parallel lines with respect to the direction of flow) were fabricated using UV- assisted capillary moulding onto glass substrate of PDMS microfluidic channel. The adhesion force of human breast epithelial cells (MCF10A) and human breast carcinoma (MCF7) was measured independently by injecting each cell line into the microfluidic device followed by culture for a period of time (e.g., one, two, and three hours). Then, the cells bound to the floor of a microfluidic channel were detached by increasing the flow rate of medium in a stepwise fashion. The adhesion force of MCF10A was always higher than that of MCF cells regardless of culture time and surface nanotopography at all flow rates, resulting in a label-free separation of cancer cells. For the cell types used in our study, the optimum separation was found for 2 hours culture on 50 nm parallel line pattern followed by flow-induced detachment at a flow rate of 300 mul/min