HISTOCHEMICAL REACTIVITY OF THE PARENCHYMA AS CRITERION OF THE MECHANISM OF ANTIHELMINTIC ACTION ON THE HOMEOSTASIS OF THE PARASITE

Abstract

Analysis of histologic specimens made from trematodes according to the generally accepted method before and after the action of anthelmintics and stained in a Schick test for the detection of glycogen, and with bromophenol blue for proteins, and toluidine blue for hexosaminoglycans using light microscopy showed that the action of the preparation in the body of trematodes caused metabolic disorders. After the action of anthelmintics, the histochemical reactivity of the connective tissue of trematodes, the parenchyma, changed. Tissue reaction to the dye increased, decreased or was absent. Weakened or disappeared metachromatic staining indicates a decrease in the amount of acid mucopolysaccharides. The Schick test demonstrates that after the action of the anthelmintic on the parasite, glycogen disappears, and the carbohydrate components are redistributed and quantitatively changed. A change in color when stained with bromophenol blue for proteins indicates a change in their nature and destruction. Histochemical analysis provides information on the quantitative and qualitative content of carbohydrate and protein compounds in the trematode parenchyma and objectively allows us to reveal the change in their ratio and nature after anthelmintics, and therefore to establish the mechanism and effect of drugs on the homeostatic disruption in the parasite's body after the host undergoes chemotherapy.