In vitro toxicity evaluation of caffeine imprinted polymer (CAF-MIP) for decaffeination method on normal chang liver cells

Abstract

Over consuming of caffeine is one of the factors to a few health problems such as insomnia, hypertensi on and cardiovascular disease. This preliminary study was conduc te to evaluate the Caffeine-Imprinted Polymer (CAF -MIP) toxicity that was synthesized for a new alternative method f or ecaffeination. It is crucial to evaluate the to xicity of CAF-MIPas this product is potential to be used as complimenta ry with any drinks containing caffeine. In this stu dy, the CAF-MIP toxicity potential was confirmed on Normal Chang Li ver cell (NCLC) based on its IC 50 value andacridine orange and propidium iodide (AO/PI) staining for mode of cell death observation.Proliferation assay was also cond ucte after 24, 48 and 72 hours at 30 μg/ml on NCLC and it showed t hat CAF-MIP promote NCLC growth as shown by at vari ous concentrationof CAF-MIPincrease the percentage of N CLC viability. Observation under light microscopes on NCLC incubated wit CAF-MIP and NIP showed the normal, v iable cell morphology, cuboidal and monolayer cell morphology and this can be seen with green fluorescence when v iew under fluorescence microscope. In conclusion, f rom this study, it is proved that the CAF-MIP does not initiate tox icity effects on human liver cells, meanwhile induc tion of cell proliferation was observed.