Identification and Characterization of Bioactive Compounds of Leaves of Justicia Gendarussa Burm. F.

Abstract

The present investigation has been carried out to find the phytochemicals present in Justicia gendarussa leaves extract. The leaves of Justicia gendarussa were collected from Kadukaval in January 2018 Thanjavur, Tamil Nadu, India. The powder leaf was extracted with aqueous and 70% methanol for 24 hours and studied for FTIR, HPLC and NMR. A preliminary phytochemical testing of the leaves extract where than to identify the phytoconstiuents, which reveals that presence of tannin, saponin, flavonoids, steroids, terpenoids, triterpenoid, carbohydrate, anthroquinone, polyphenol, glycoside bioactive compound was isolated by column chromatography technique. The collected flavonoid fractions was purified by thin layer chromatography. FTIR, HPLC and NMR studies were carried out to find the structure of bioactive compound Quercetin. 1 HNMR and 13 C – NMR that reveals the structure of flavonoids. The compound was identified as 3, 3’, 4’, 5, 7 – pentahydroxyflavanone by 1 HNMR and 13 C – NMR. All these data obtained in the present investigation supported the rich source of phytochemicals preent in J.gendarussa leaves extract and therby traditional claim associated with J. gendarussa literature. KeywordsJusticia gendarussa, phytochemical, FTIR, HPLC and NMR