PERBANDINGAN HIDROLISIS ENZIMATIS DAN ASAM TERHADAP PATI JAGUNG MANIS (Zea mays L.) DALAM PEMBUATAN GULA CAIR

JURNAL SAINS NATURAL Pub Date : 2019-12-14 DOI:10.31938/JSN.V7I2.255

Rtm Sutamihardja, Mia Azizah, Bekti Dwisepti Mafiana

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引用次数: 1

Abstract

Comparison Hydrolisis of Enzymatic and Acid of Sweet Corn Starch (Zea mays L.) in Liquid Sugar ProductionSweet corn starch (Zea mays L.) contains high carbohydrate that can be used for food and industrial purposes. Sweet corn starch can be used for liquid sugar as alternative sweetener by enzymatic or acid hydrolysis. The enzymatic hydrolysis was performed by treating the starch with alpha amylase for liquefaction and glucoamylase for saccharification, while acid hydrolysis was performed by mixing the starch with 1,0 N hydrochloric acid. According to the results, the yield of enzymatic hydrolysis is higher than acid hydrolysis. The highest yield of liquid sugar is 91,73% produced by enzymatic hydrolysis using alpha amylase 42µL and glucoamylase 42µL and 59,40% of reducing sugar. The yield of liquid sugar produced by acid hydrolysis using HCl 1,0 N is 78,55% and 31,48% of reducing sugar.Key words: Zea mays, starch, liquid sugar, hydrolisis of hydrochloric acid, hydrolisis of alpha amylase, hydrolisis of glucoamylaseABSTRAKPati jagung manis (Zea mays L.) mengandung karbohidrat cukup tinggi yang dapat dimanfaatkan untuk bahan pangan dan industri. Pati jagung manis dapat diolah menjadi gula cair dan digunakan sebagai pemanis alternatif melalui hidrolisis pati baik secara enzimatis atau asam. Hidrolisis enzimatis melalui tahap likuifikasi menggunakan alfa amilase dan tahap sakarifikasi menggunakan glukoamilase. Hidrolisis asam dilakukan menggunakan asam klorida 0,1 N. Hasil penelitian menunjukkan bahwa gula cair hasil hidrolisis enzimatis menghasilkan rendemen lebih tinggi dibandingkan hidrolisis asam. Rendemen gula cair paling tinggi dihasilkan pada proses hidrolisis enzimatis menggunakan alfa amilase 42µL dan glukoamilase 42µL sebesar 91,73% dengan nilai gula pereduksi sebesar 59,40%. Rendemen gula cair hidrolisis asam menggunakan HCl 1,0 N sebesar 78,55% dengan nilai gula pereduksi sebesar 31,48%.Kata kunci: Zea mays, pati, gula cair, hidrolisis HCl, hidrolisis enzim alfa-amilase, hidrolisis enzim glukoamilase

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酶化和酸对甜玉米淀粉的比例(Zea mays L)。在液态糖的制造中

蔗糖生产中甜玉米淀粉(Zea mays L.)酶解和酸解的比较甜玉米淀粉(Zea mays L.)含有高碳水化合物，可用于食品和工业用途。甜玉米淀粉可以通过酶解或酸水解作为替代甜味剂用于液体糖。酶解采用α淀粉酶液化和葡萄糖淀粉酶糖化的方法，酸解采用1,0 N盐酸混合的方法。结果表明，酶水解的产率高于酸水解。采用α淀粉酶42µL和葡萄糖淀粉酶42µL和还原糖59.40%的酶解法，液体糖的最高产率为91.73%。盐酸1.0 N酸水解液糖的产率分别为还原糖的78.55%和31.48%。关键词:玉米，淀粉，液体糖，盐酸水解，α -淀粉酶水解，葡萄糖淀粉酶水解Pati jagung manis dapat diolah menjadi gula cair dan digunakan sebagai pmanis alternatialaluhidolis Pati baik secara enzimatis atau asam。蒙古那坎α淀粉酶和蒙古那坎葡萄糖淀粉酶。不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹不丹Rendemen咽喉以下简称木栅丁宜受困dihasilkan篇散文hidrolisis enzimatis menggunakan阿尔法amilase 42µL丹glukoamilase 42µL sebesar 91, 73% dengan汝咽喉pereduksi sebesar 59岁的40%。renmen gula椅子出汗asam menggunakan HCl 1,0, N sebesar 78,55%， dengan nilai gula pereduksi sebesar 31,48%。Kata kunci:玉米，木瓜，古拉木，水解HCl，水解酶α淀粉酶，水解酶葡萄糖淀粉酶

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