Electrophoretic Karyotypes of Fusarium spp.

Experimental Mycology Pub Date : 1993-12-01 DOI:10.1006/emyc.1993.1031

Quirico Migheli, Tilde Berio, M.Lodovica Gullino

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引用次数: 41

Abstract

Migheli, Q., Berio, T., and Gullino, M. L. 1993. Electrophoretic karyotypes of Fusarium spp. Experimental Mycology 17, 329-337. The electrophoretic karyotype of 17 antagonistic and pathogenic strains of Fusarium spp. has been established by using contour-clamped homogeneous electric field gel electrophoresis. Intact chromosomal DNA was prepared from fungal protoplasts with standard procedures. Up to 11 distinct chromosomal bands were resolved after 184 h of migration at 50 V. Polymorphic karyotypes were observed in different species of Fusarium, formae speciales of F. oxysporum , and races of F. oxysporum f.sp. dianthi. Using the Schizosaccharomyces pombe and Saccharomyces cerevisiae chromosomes as size standards, the size of the Fusarium genome was estimated to range from approximately 18.1 to 51.5 Mb. The suitability of electrophoretic karyotyping as a tool for strain characterization, as well as some applications in hybridization analysis of Fusarium spp., is discussed.

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镰刀菌的电泳核型。

米格里，Q.，贝里奥，T.和古利诺，M. L. 1993。镰刀菌的电泳核型。实验真菌学17,329-337。采用等边夹持均匀电场凝胶电泳技术，建立了17株镰刀菌拮抗和致病性菌株的电泳核型。用标准程序从真菌原生质体中制备完整的染色体DNA。在50v下迁移184 h后，可分离出11条不同的染色体带。镰刀菌不同种、尖孢镰刀菌不同种、尖孢镰刀菌不同种、尖孢镰刀菌不同小种均存在多态核型。dianthi。以裂糖酵母(Schizosaccharomyces pombe)和酿酒酵母(Saccharomyces cerevisiae)的染色体为标准，估计镰刀菌基因组的大小在18.1 ~ 51.5 Mb之间。本文讨论了电泳核型作为菌株鉴定工具的适用性，以及在镰刀菌杂交分析中的一些应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Experimental Mycology

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