СРАВНИТЕЛЬНЫЙ АНАЛИЗ ПАТОГЕННОСТИ ВИРУСОВ ГРИППА A(H5N1) И A(H1N1)PDM09 У ЛАБОРАТОРНЫХ МЫШЕЙ

E. A. Prokopeva, K. A. Sharshov, Anna Romanovskaya, I. Sobolev, Olga Kurskaya, E. I. Soloveva, Lidiya V. Shestopalova, A. V. Zaykovskaya, A. Alekseev, A. M. Shestopalov
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Abstract

Aim. Compare the degree of pathogenicity of newly emerging influenza virus strains with pandemic potential by experimental infection of BALB/c mice with highly pathogenic avian influenza A(H5N1) virus and mouse adapted pandemic influenza A(H1N1)pdm09 virus. Materials and methods. The first group of BALB/c mice (n=24) were infected with highly pathogenic avian influenza A(H5N1) virus in a dose of 5 LD50, and the second group (n=24) were infected with mouse adapted pandemic influenza A(H1N1)pdm09 virus at a dose of 5 LD50. Determination of LD50 and TCID50 were performed by virological methods. Morphological changes in internal organs (lung, brain, liver, kidney, spleen) were examined by light and transmission electron microscopy. Results. Virologic analysis showed that both strains are highly lethal for mice. Microscopic examination revealed the development of interstitial pneumonia in the lungs and generalization of infection in the internal organs. Conclusion. In both groups of BALB/c mice experimentally infected with highly pathogenic avian influenza A(H5N1) and mouse adapted pandemic influenza A(H1N1)pdm09 virus were revealed the development of a highly lethal disease in the form of respiratory pneumonia. It is noted, different mechanisms of pathological process: in case of infection with mouse adapted pandemic influenza A(H1N1)pdm09 virus firstly developed bronchitis, which quickly exacerbated by the development of alveolitis, while in case of infection with highly pathogenic avian influenza A(H5N1) immediately developed alveolitis. On the 6th day after infection recorded the development of generalized infection in mice of both experimental groups.
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的目标。通过BALB/c小鼠实验感染高致病性H5N1禽流感病毒和小鼠适应型甲型H1N1大流行性流感pdm09病毒,比较具有大流行潜力的新出现流感病毒毒株的致病性程度。材料和方法。第一组BALB/c小鼠(n=24)以5 LD50剂量感染高致病性甲型H5N1禽流感病毒,第二组小鼠(n=24)以5 LD50剂量感染小鼠适应型甲型H1N1大流行性流感pdm09病毒。采用病毒学方法测定LD50和TCID50。光镜及透射电镜观察肺、脑、肝、肾、脾等脏器形态学变化。结果。病毒学分析表明,这两种毒株对小鼠具有高致死率。显微镜检查显示肺间质性肺炎的发展和内脏器官感染的普遍化。结论。在实验感染高致病性甲型H5N1禽流感和小鼠适应性甲型H1N1大流行性流感pdm09病毒的两组BALB/c小鼠中,均出现以呼吸道肺炎形式出现的高致命性疾病。值得注意的是,不同的病理过程机制:当感染小鼠适应性大流行性流感A(H1N1)pdm09病毒时,首先发展为支气管炎,并迅速加重发展为肺泡炎,而当感染高致病性甲型禽流感(H5N1)时,立即发展为肺泡炎。感染后第6天记录两组小鼠全身性感染的发生情况。
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来源期刊
Zhurnal mikrobiologii, epidemiologii, i immunobiologii
Zhurnal mikrobiologii, epidemiologii, i immunobiologii Immunology and Microbiology-Immunology and Microbiology (miscellaneous)
CiteScore
1.40
自引率
0.00%
发文量
51
审稿时长
8 weeks
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