Highly selective and sensitive DNA measurement by microelectrical impedance spectroscopy

The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05. Pub Date : 2005-06-05 DOI:10.1109/SENSOR.2005.1497322

Sang Wook Lee, T. Yamamoto, Teruo Fujii

{"title":"Highly selective and sensitive DNA measurement by microelectrical impedance spectroscopy","authors":"Sang Wook Lee, T. Yamamoto, Teruo Fujii","doi":"10.1109/SENSOR.2005.1497322","DOIUrl":null,"url":null,"abstract":"A novel /spl mu/-EIS (microelectrical impedance spectroscopy) device for label free analysis of DNA are developed and experimentally demonstrated. The feature of /spl mu/-EIS system is not only detecting DNA without any labelling, but also controlling the conformation that would enhance the EIS signal. The /spl mu/-EIS system is fully integrated with a microfluidic network made of polydimethylsiloxane (PDMS), a micromeasurement electrode and a micropump in order to conduct a semiautomated measurement controlled by an external PC system. The micro EIS system is capable of detecting the DNA concentration of a few nM in aqueous solution of a few pl in volume by virtue of the conformation enhanced impedance response. As a first demonstration of conformational change induced DNA analysis, the frequency and the electric field strength dependence of various length and concentrations of DNA were evaluated.","PeriodicalId":22359,"journal":{"name":"The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.","volume":"20 1","pages":"1314-1317 Vol. 2"},"PeriodicalIF":0.0000,"publicationDate":"2005-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/SENSOR.2005.1497322","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 2

Abstract

A novel /spl mu/-EIS (microelectrical impedance spectroscopy) device for label free analysis of DNA are developed and experimentally demonstrated. The feature of /spl mu/-EIS system is not only detecting DNA without any labelling, but also controlling the conformation that would enhance the EIS signal. The /spl mu/-EIS system is fully integrated with a microfluidic network made of polydimethylsiloxane (PDMS), a micromeasurement electrode and a micropump in order to conduct a semiautomated measurement controlled by an external PC system. The micro EIS system is capable of detecting the DNA concentration of a few nM in aqueous solution of a few pl in volume by virtue of the conformation enhanced impedance response. As a first demonstration of conformational change induced DNA analysis, the frequency and the electric field strength dependence of various length and concentrations of DNA were evaluated.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

高选择性和灵敏度的DNA测量微电阻抗谱

研制了一种新型的/spl μ /-EIS(微电阻抗谱)装置，并进行了实验验证。/spl mu/-EIS系统的特点是不仅可以不加标记地检测DNA，而且可以控制增强EIS信号的构象。/spl mu/-EIS系统完全集成了由聚二甲基硅氧烷(PDMS)，微测量电极和微泵组成的微流控网络，以便通过外部PC系统控制进行半自动测量。微EIS系统能够在体积为几pl的水溶液中检测到几nM的DNA浓度，这是由于其构象增强阻抗响应。作为构象变化诱导DNA分析的第一个演示，我们评估了不同长度和浓度的DNA的频率和电场强度依赖性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

The 13th International Conference on Solid-State Sensors, Actuators and Microsystems, 2005. Digest of Technical Papers. TRANSDUCERS '05.

自引率

0.00%

发文量

期刊最新文献

A new capacitive displacement sensor with high accuracy and long range Novel NDIR CO/sub 2/ sensor for indoor air quality monitoring A thin film strip for aerodynamic body pressure profile monitoring High power electrostatic motor with micropatterned electret on shrouded turbine Implantable blood pressure monitoring of small animal for advanced biological research