Inhibition of Gene Expression of Trehalase Enhances Drought Resistance in Transgenic Tobacco

2010 4th International Conference on Bioinformatics and Biomedical Engineering Pub Date : 2010-06-18 DOI:10.1109/ICBBE.2010.5516954

Bei Guo, Y. Song, Xuefeng Guo, Wenping Wang, Ning Ding

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引用次数: 3

Abstract

The representative individuals, CK (non-transgenic), 121 (with pBI121 vector), 1285 (with pGSA1285 vector), T4 (with gene of trehalase, TRE), S1 (with gene of trehalose -6 - phosphate synthase, TPS), P3 (with gene of trehalose -6 - phosphate phosphatase, TPP), a3 (with anti-TRE gene), i2 (with RNAi-TRE gene), were selected for identification of drought resistance, and the Malondialdehyde (MDA), relative conductivity have been measured after water-stress during this experiment. The results showed that the transgenic tobaccos with anti-TRE and RNAi-TRE grew better than other individuals under water-stress conditions; conductivity of i2 was lower than other plants. Meantime, i2 has performance of some special features. These indicate that through application the antisense RNA and RNA interference technology to trehalase gene, it is possible to make the receptor trehalose metabolism changes. The inhibition of gene expression of trehalase can led to the accumulation of trehalose content and the enhancement of drought resistance in the transgenic tobaccos.

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抑制海藻糖酶基因表达增强转基因烟草抗旱性

选择代表性个体CK(非转基因)、121(携带pBI121载体)、1285(携带pGSA1285载体)、T4(携带海藻糖酶基因TRE)、S1(携带海藻糖-6 -磷酸合酶基因TPS)、P3(携带海藻糖-6 -磷酸合酶基因TPP)、a3(携带抗TRE基因)、i2(携带RNAi-TRE基因)进行抗旱性鉴定，并在本试验中测定了胁迫后丙二醛(MDA)的相对电导率。结果表明，抗- tre和RNAi-TRE转基因烟草在水分胁迫条件下的生长情况优于其他个体;i2的电导率低于其他植株。同时，i2具有一些特殊的性能。这说明通过对海藻糖酶基因应用反义RNA和RNA干扰技术，有可能使受体海藻糖代谢发生改变。抑制海藻糖酶基因表达可使转基因烟草海藻糖含量积累，抗旱性增强。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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2010 4th International Conference on Bioinformatics and Biomedical Engineering

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