Asphyxia effects on colony forming ability of hematopoietic stem cell of cord blood

Abstract

Background:Asphyxia is the medical situation resulting of deprivation of oxygen to a newborn infant that lasts long enough during the birth process to cause physical harm usually to the brain. Human umbilical cord blood (UCB) is a well-established source of hematopoietic stem/progenitor cells (HSPCs) for allogeneic stem cell transplantation. Low level of O2 in infants with asphyxia during labor can influence on proliferation and differentiation of stem cells in cord blood. Method:The quality and colony forming unit of hematopoietic stem cells in cord blood of infants with severe asphyxia with Apgar score 3-5or need to cardiac pulmonary resuscitation five minutes after delivery were compared with the normal group with normal Apgar score.Afterward, hematopoietic stem cells were isolated and cells were cultured in enrichedmedia (MethoCult H4435) special for HSPCs for 7 days to assessment growth and colony formation. Results: There was a significant difference in the number of RBC (P=0.0016) and WBC precursor’s colonies (P = 0.006), in plate with 104 cord blood hematopoietic stem cells in infants who exposed to hypoxemia during labor.Conclusion: Umbilical cord blood is valued for its content of stem cells.Severehypoxia in the perinatal period does not have negative influence on viability of UCB hematopoietic stem cells to growth and formation colonies.Based on our research transient severe asphyxia does not have negative effects on HSPCs to save and banking for likely problems in future