Isolation and Multiple Drug Resistance Patterns of Salmonella Isolates from Selected Dairy Farms in Hawassa Town, Ethiopia

Abstract

A cross-sectional study was conducted from November 2017 to May 2018 on selected dairy farms in Hawassa town to isolate and assess the in-vitro antibiotic biogram of Salmonella from lactating dairy cows, personnel’s and equipments at farms. A total of 216 samples were collected from selected dairy farms. All samples were processed bacteriologically following standard procedures outlined by ISO 6579: 2002. The overall prevalence of Salmonella was 12.9% (28/216) of the total samples. Out of total, 64.3% (18/28), 10.7% (3/28) and 25% (7/28) were from lactating cows, personnels’, and equipments, respectively. Based on antimicrobial susceptibility testing, all isolates were resistant at least to one or more antimicrobials tested. Accordingly, 96.4% (27/28), 82.1% (23/28) and 75.0% (21/28) isolates showed resistance for oxytetracycline, kanamycin, and nalidixic acid, respectively. Out of all the resistant isolates, 96.4% (27/28) showed multiple antibiotic resistance (resistance to two or more antibiotics) patterns. Multiple antimicrobials resistance was observed in 66.7% (18/27), 7.4% (2/27) and 25.9% (7/27) from lactating cows, personnels’, and equipments, respectively. Thus, awareness creation to the public regarding the public health importance of multiple drug-resistant Salmonella species and the consumption of unpasteurized milk and milk products is important. Fesseha H*, Aliye S, Kifle T and Mathewos M School of Veterinary Medicine, Wolaita Sodo University, Ethiopia *Address for correspondence: Haben Fesseha, School of Veterinary Medicine, Wolaita Sodo University, Ethiopia, PO Box 138, Wolaita Sodo, Southern, Ethiopia; E-mail: tseyon.h@gmail.com Submission: 20-January-2020 Accepted: 21-February-2020 Published: 22February-2020 Copyright: © 2020 Fesseha H Heredia Peralta DT, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Research Article Open Access