Antiinflammatory and ROS Suppressive Effects of the Addition of Fiber to a High-Fat High-Calorie Meal

H. Ghanim, M. Batra, S. Abuaysheh, K. Green, A. Makdissi, Nitesh D Kuhadiya, A. Chaudhuri, P. Dandona
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引用次数: 29

Abstract

Background Fiber intake is associated with a reduction in the occurrence of cardiovascular events and diabetes. Objective To investigate whether the addition of fiber to a high-fat, high-calorie (HFHC) meal prevents proinflammatory changes induced by the HFHC meal. Design Ten normal fasting subjects consumed an HFHC meal with or without an additional 30 g of insoluble dietary fiber on 2 separate visits. Blood samples were collected over 5 hours, and mononuclear cells (MNCs) were isolated. Results Fiber addition to the HFHC meal significantly lowered glucose excursion in the first 90 minutes and increased insulin and C-peptide secretion throughout the 5-hour follow-up period compared with the meal alone. The HFHC meal induced increases in lipopolysaccharide (LPS) concentrations, MNC reactive oxygen species generation, and the expression of interleukin (IL)-1β, tumor necrosis factor α (TNF-α), Toll-like receptor (TLR)-4, and CD14. The addition of fiber prevented an increase in LPS and significantly reduced the increases in ROS generation and the expression of IL-1β, TNF-α, TLR-4, and CD14. In addition, the meal increased Suppressor of cytokine signaling (SOCS)-3 and protein tyrosine phosphatase 1B (PTP-1B) messenger RNA and protein levels, which were inhibited when fiber was added. Conclusions The addition of fiber to a proinflammatory HFHC meal had beneficial anti-inflammatory and metabolic effects. Thus, the fiber content of the American Heart Association meal may contribute to its noninflammatory nature. If these actions of dietary fiber are sustained following long-term intake, they may contribute to fiber's known benefits in the prevention of insulin resistance, type 2 diabetes, and atherosclerosis.
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高脂肪高热量膳食中添加纤维的抗炎和ROS抑制作用
背景:纤维摄入与减少心血管事件和糖尿病的发生有关。目的探讨在高脂高热量膳食中添加纤维是否能预防高脂高热量膳食引起的促炎改变。10名正常禁食的受试者分别在两次访问中食用含或不含30g不溶性膳食纤维的HFHC餐。5小时采集血样,分离单核细胞(MNCs)。结果与单独膳食相比,在HFHC膳食中添加纤维可显著降低前90分钟的葡萄糖漂移,并在整个5小时的随访期间增加胰岛素和c肽分泌。HFHC膳食诱导脂多糖(LPS)浓度、MNC活性氧生成以及白细胞介素(IL)-1β、肿瘤坏死因子α (TNF-α)、toll样受体(TLR)-4和CD14的表达增加。纤维的添加阻止了LPS的增加,并显著降低了ROS生成的增加以及IL-1β、TNF-α、TLR-4和CD14的表达。此外,膳食增加了细胞因子信号抑制因子(SOCS)-3和蛋白酪氨酸磷酸酶1B (PTP-1B)信使RNA和蛋白水平,而添加纤维则抑制了这些水平。结论在促炎的HFHC餐中添加纤维具有良好的抗炎和代谢作用。因此,美国心脏协会膳食的纤维含量可能有助于其非炎症性。如果膳食纤维的这些作用在长期摄入后持续下去,它们可能有助于纤维在预防胰岛素抵抗、2型糖尿病和动脉粥样硬化方面的已知益处。
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