{"title":"Notice of RetractionKinetic Studies for the Inhibition Effect of 4-Chlorophenol on Acetylcholinesterase Activity","authors":"Q. Liu, Jun-xia Liu","doi":"10.1109/ICBBE.2011.5781411","DOIUrl":null,"url":null,"abstract":"The inhibition effect of 4-chlorophenol (4-CP) on acetylcholinesterase (AChE) and its kinetics were studied by spectrophotometry. The results show that the activity of AChE can be inhibited obviously by 4-CP, and the 50% inhibitory concentration (IC<sub>50</sub>) is 0.66 mmol·L<sup>-1</sup>. 4-CP acted as a mixed inhibitor of AChE, and a reversible inhibition mechanism were observed. Lineweaver-Burk plot results showed that the mixed inhibitory constants K<sub>i</sub> and K'<sub>i</sub> were 0.55 mmol·L<sup>-1</sup> and 1.60 mmol·L<sup>-1</sup>, respectively, which indicated that the inhibition effect of 4-CP on free enzyme was stronger than that on enzyme-substrate complex.","PeriodicalId":6438,"journal":{"name":"2011 5th International Conference on Bioinformatics and Biomedical Engineering","volume":"180 1","pages":"1-4"},"PeriodicalIF":0.0000,"publicationDate":"2011-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"2011 5th International Conference on Bioinformatics and Biomedical Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/ICBBE.2011.5781411","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
The inhibition effect of 4-chlorophenol (4-CP) on acetylcholinesterase (AChE) and its kinetics were studied by spectrophotometry. The results show that the activity of AChE can be inhibited obviously by 4-CP, and the 50% inhibitory concentration (IC50) is 0.66 mmol·L-1. 4-CP acted as a mixed inhibitor of AChE, and a reversible inhibition mechanism were observed. Lineweaver-Burk plot results showed that the mixed inhibitory constants Ki and K'i were 0.55 mmol·L-1 and 1.60 mmol·L-1, respectively, which indicated that the inhibition effect of 4-CP on free enzyme was stronger than that on enzyme-substrate complex.
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