Cloning and nucleotide sequencing of genes encoding Mn-superoxide dismutase and class II fumarase from Thermus aquaticus YT-1

Hidemasa Motoshima , Etsuo Minagawa, Fuji Tsukasaki, Shuichi Kaminogawa
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引用次数: 6

Abstract

A 6955-bp sequence of a PstI-HindIII DNA fragment containing the manganese-superoxide dismutase (MnSOD) gene of Thermus aquaticus YT-1 was determined. The gene (sod) encoded a polypeptide consisting of 204 amino acid residues (the mature enzyme without the initiation methionine) with a calculated Mr of 22,773. The deduced amino acid sequence of the sod gene showed 92% identity to that of Thermus thermophilus HB8 determined chemically. The sod gene was well expressed in Escherichia coli and a heat-stable active enzyme was produced. An open reading frame encoding fumarase was found 91 bp upstream of the MnSOD gene in tandem form. The fum gene encoded a polypeptide consisting of 466 amino acid residues with a calculated Mr of 50,950. The deduced amino acid sequence of the fum gene product has similarity to that of the fumC of E. coli (57% identity), suggesting that this gene encodes a class II type fumarase.

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水热菌mn -超氧化物歧化酶和II类富马酸酶基因的克隆与序列分析
测定了一段含锰超氧化物歧化酶(MnSOD)基因的水热菌(Thermus aquaticus) pti - hindiii DNA片段6955 bp的序列。该基因(sod)编码一个由204个氨基酸残基组成的多肽(不含起始蛋氨酸的成熟酶),计算Mr为22,773。sod基因的氨基酸序列与嗜热热菌HB8的同源性达到92%。sod基因在大肠杆菌中得到了良好的表达,并产生了一种热稳定的活性酶。在MnSOD基因上游91 bp处发现了一个编码富马酸酶的开放阅读框。真菌基因编码了一个由466个氨基酸残基组成的多肽,计算Mr为50,950。推导出的富马酸基因产物的氨基酸序列与大肠杆菌的富马酸相似(同源性为57%),表明该基因编码ⅱ类富马酸酶。
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