Difference mid-IR nanospectroscopy on individual patches of purple membranes: the proton pump activity of bacteriorhodopsin at the nanoscale

V. Giliberti, R. Polito, E. Ritter, M. Broser, P. Hegemann, L. Puskar, U. Schade, L. Zanetti‐Polzi, I. Daidone, S. Corni, F. Rusconi, P. Biagioni, L. Baldassarre, M. Ortolani
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Abstract

Conformational changes of the light-sensitive membrane receptor and proton pump bacteriorhodopsin is investigated by tip-enhanced mid-infrared nanospectroscopy on individual cell membrane patches, with a dramatic increase of sensitivity (in terms of number of probed molecules) compared with standard mid-infrared spectroscopy.
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