PFGE XbaI® Indistinguishable properties of Salmonella Kastrup and Salmonella Larochelle isolates at beef processing and distribution continuum

A. Hiko, G. Agga, L. Bräutigam, H. Irsigler, G. Ameni, Fries Reinhard
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Abstract

Salmonellosis is a major cause of foodborne illnesses in humans throughout the world. Pulsed field gel electrophoresis (PFGE) is a commonly used tool for epidemiological survey and source tracking of Salmonella strains isolated from various sources. A total of 237 samples from various sources were collected at abattoir and butcheries in Ethiopia from December 2011 to April 2012. Isolates of Salmonella Kastrup (n = 3) and Salmonella Larochelle (n = 11) were characterized for antimicrobial resistance using disc diffusion and for genotypic profiles using PFGE XbaI®. Salmonella Kastrup and S. Larochelle were isolated from 1.3% and 4.6% of total samples (n = 237) examined. Except for water samples (n = 12) and truck swabs (n=11), all other sample sources were positive for one or more of S. Kastrup and S. Larochelle . Salmonella Kastrup was isolated in Ethiopia for the first time. Salmonella isolates from both Kastrup and Larochelle serotypes showed similar antimicrobial resistance profiles. Two pulsotypes consisting of the same three S. Kastrup and 10 S. Larochelle cluster and second cluster consisting of one S. Larochelle were identified at 1.5% and 1% band positions tolerance by unweighted-pair group method (UPGM) and showed ≥91% similarity. S. Kastrup and S. Larochelle isolates showed similar antimicrobial resistance profiles with indistinguishable PFGE XbaI® patterns indicating possible clonal spread of the isolates along beef processing and distribution continuum. Key words : Salmonella ; PFGE; Antimicrobial resistance; Beef; Ethiopia
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PFGE XbaI®在牛肉加工和分销连续体中分离的卡鲁普沙门氏菌和拉罗谢尔沙门氏菌的难以区分特性
沙门氏菌病是全世界人类食源性疾病的一个主要原因。脉冲场凝胶电泳(PFGE)是一种常用的流行病学调查和来源追踪工具。从2011年12月至2012年4月,在埃塞俄比亚的屠宰场和屠宰场共收集了237个不同来源的样本。分离菌株Kastrup沙门氏菌(n = 3)和Larochelle沙门氏菌(n = 11)分别采用盘扩散法和PFGE XbaI®法进行耐药性鉴定和基因型分析。卡斯特鲁普沙门氏菌和拉罗谢尔沙门氏菌分离率分别为1.3%和4.6% (n = 237)。除水样(n= 12)和卡车拭子(n=11)外,所有其他样本来源均对一种或多种S. Kastrup和S. Larochelle呈阳性。在埃塞俄比亚首次分离出卡斯特鲁普沙门氏菌。从Kastrup和Larochelle血清型分离的沙门氏菌显示出相似的抗菌素耐药谱。采用非加权对群法(UPGM)分别鉴定出3个S. Kastrup和10个S. Larochelle和1个S. Larochelle组成的2个脉冲型和1个S. Larochelle组成的2个脉冲型,相似度≥91%。S. Kastrup和S. Larochelle分离株显示出相似的抗菌耐药谱,PFGE XbaI®模式难以区分,表明分离株可能沿着牛肉加工和分布连续体克隆传播。关键词:沙门氏菌;但是脉冲场凝胶电泳的出现;抗菌素耐药性;牛肉;埃塞俄比亚
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