OBTAINING STANDARD HELMINTH EGG CULTURES FOR STUDYING THE OVICIDAL EFFICACY OF CHEMICALS

Abstract

In the prevention of parasitic diseases, an important place is occupied by the search for new highly effective chemicals for the disinfection of environmental objects. When selecting disinfectants, helminth eggs are often used as test objects. The availability of high-quality and standard test material contributes greatly to successful research. However, publications devoted to the methodological side of this problem are clearly not enough. Ascaridata eggs (Ascaris suum, Toxocara canis, etc.) are the most resistant to chemicals, so testing on them allows us to identify the most effective means for the purposes of disinfection. Special attention should be paid to such an object as Enterobius vermicularis, the causative agent of enterobiasis, the most common of helminth infections in humans. This paper describes methods for obtaining standard egg cultures of such test objects as A. suum and E. vermicularis. A more standard A. suum egg culture is facilitated by material collected only from females with high egg viability, as well as the destruction of egg conglomerates in a suspension using sodium hypochlorite. Standardization of the E. vermicularis egg culture is only achieved by taking samples containing healthy viable eggs after the preview.